2int
From Proteopedia
(New page: 200px<br /> <applet load="2int" size="450" color="white" frame="true" align="right" spinBox="true" caption="2int, resolution 2.35Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:2int.gif|left|200px]]<br /> | + | [[Image:2int.gif|left|200px]]<br /><applet load="2int" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2int" size=" | + | |
caption="2int, resolution 2.35Å" /> | caption="2int, resolution 2.35Å" /> | ||
'''CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-4'''<br /> | '''CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-4'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of recombinant human interleukin-4 (rhuIL-4) was | + | The crystal structure of recombinant human interleukin-4 (rhuIL-4) was initially determined at 3.5-A resolution by multiple isomorphous replacement techniques and subsequently refined to a resolution of 2.35 A by simulated annealing. The final crystallographic R-factor, based on all data in the range 6.0-2.35 A (7470 reflections), is 0.232. Bond lengths and bond angles in the molecule have root mean square deviations from ideal values of 0.016 A and 2.4 degrees, respectively. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhuIL-4 is a four alpha-helix bundle, which composes approximately 58% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within these connections is a two-stranded antiparallel beta-sheet. Both the tertiary and secondary structures of rhuIL-4 are similar to those of human granulocyte-macrophage colony-stimulating factor. Critical regions for receptor binding are proposed. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2INT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2INT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bugg, C | + | [[Category: Bugg, C E.]] |
| - | [[Category: Cook, W | + | [[Category: Cook, W J.]] |
| - | [[Category: Ealick, S | + | [[Category: Ealick, S E.]] |
| - | [[Category: Junior, R | + | [[Category: Junior, R Cameron.]] |
| - | [[Category: Junior, R | + | [[Category: Junior, R L.Walter.]] |
| - | [[Category: Nagabhushan, T | + | [[Category: Nagabhushan, T L.]] |
[[Category: Reichert, P.]] | [[Category: Reichert, P.]] | ||
| - | [[Category: Trotta, P | + | [[Category: Trotta, P P.]] |
| - | [[Category: Walter, M | + | [[Category: Walter, M R.]] |
| - | [[Category: Zhao, B | + | [[Category: Zhao, B G.]] |
[[Category: cytokine]] | [[Category: cytokine]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:54:21 2008'' |
Revision as of 15:54, 21 February 2008
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CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-4
Contents |
Overview
The crystal structure of recombinant human interleukin-4 (rhuIL-4) was initially determined at 3.5-A resolution by multiple isomorphous replacement techniques and subsequently refined to a resolution of 2.35 A by simulated annealing. The final crystallographic R-factor, based on all data in the range 6.0-2.35 A (7470 reflections), is 0.232. Bond lengths and bond angles in the molecule have root mean square deviations from ideal values of 0.016 A and 2.4 degrees, respectively. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhuIL-4 is a four alpha-helix bundle, which composes approximately 58% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within these connections is a two-stranded antiparallel beta-sheet. Both the tertiary and secondary structures of rhuIL-4 are similar to those of human granulocyte-macrophage colony-stimulating factor. Critical regions for receptor binding are proposed.
Disease
Known diseases associated with this structure: AIDS, slow progression to OMIM:[147781], Atopy, susceptibility to OMIM:[147781]
About this Structure
2INT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of recombinant human interleukin-4., Walter MR, Cook WJ, Zhao BG, Cameron RP Jr, Ealick SE, Walter RL Jr, Reichert P, Nagabhushan TL, Trotta PP, Bugg CE, J Biol Chem. 1992 Oct 5;267(28):20371-6. PMID:1400355
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