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2f3j

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[[Image:2f3j.jpg|left|200px]]
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{{STRUCTURE_2f3j| PDB=2f3j | SCENE= }}
{{STRUCTURE_2f3j| PDB=2f3j | SCENE= }}
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'''The solution structure of the REF2-I mRNA export factor (residues 1-155).'''
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===The solution structure of the REF2-I mRNA export factor (residues 1-155).===
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==Overview==
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The RNA binding and export factor (REF) family of mRNA export adaptors are found in several nuclear protein complexes including the spliceosome, TREX, and exon junction complexes. They bind RNA, interact with the helicase UAP56/DDX39, and are thought to bridge the interaction between the export factor TAP/NXF1 and mRNA. REF2-I consists of three domains, with the RNA recognition motif (RRM) domain positioned in the middle. Here we dissect the interdomain interactions of REF2-I and present the solution structure of a functionally competent double domain (NM; residues 1-155). The N-terminal domain comprises a transient helix (N-helix) linked to the RRM by a flexible arm that includes an Arg-rich region. The N-helix, which is required for REF2-I function in vivo, overlaps the highly conserved REF-N motif and, together with the adjacent Arg-rich region, interacts transiently with the RRM. RNA interacts with REF2-I through arginine-rich regions in its N- and C-terminal domains, but we show that it also interacts weakly with the RRM. The mode of interaction is unusual for an RRM since it involves loops L1 and L5. NMR signal mapping and biochemical analysis with NM indicate that DDX39 and TAP interact with both the N and RRM domains of REF2-I and show that binding of these proteins and RNA will favor an open conformation for the two domains. The proximity of the RNA, TAP, and DDX39 binding sites on REF2-I suggests their binding may be mutually exclusive, which would lead to successive ligand binding events in the course of mRNA export.
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(as it appears on PubMed at http://www.pubmed.gov), where 17000901 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17000901}}
==About this Structure==
==About this Structure==
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2F3J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3J OCA].
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2F3J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3J OCA].
==Reference==
==Reference==
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[[Category: Rbd domain.]]
[[Category: Rbd domain.]]
[[Category: Rrm domain]]
[[Category: Rrm domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:25:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:02:27 2008''

Revision as of 10:02, 29 July 2008

Image:2f3j.png

Template:STRUCTURE 2f3j

The solution structure of the REF2-I mRNA export factor (residues 1-155).

Template:ABSTRACT PUBMED 17000901

About this Structure

2F3J is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

The solution structure of REF2-I reveals interdomain interactions and regions involved in binding mRNA export factors and RNA., Golovanov AP, Hautbergue GM, Tintaru AM, Lian LY, Wilson SA, RNA. 2006 Nov;12(11):1933-48. Epub 2006 Sep 25. PMID:17000901

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