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2f54

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{{STRUCTURE_2f54| PDB=2f54 | SCENE= }}
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'''Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity'''
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===Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity===
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==Overview==
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The mammalian alpha/beta T cell receptor (TCR) repertoire plays a pivotal role in adaptive immunity by recognizing short, processed, peptide antigens bound in the context of a highly diverse family of cell-surface major histocompatibility complexes (pMHCs). Despite the extensive TCR-MHC interaction surface, peptide-independent cross-reactivity of native TCRs is generally avoided through cell-mediated selection of molecules with low inherent affinity for MHC. Here we show that, contrary to expectations, the germ line-encoded complementarity determining regions (CDRs) of human TCRs, namely the CDR2s, which appear to contact only the MHC surface and not the bound peptide, can be engineered to yield soluble low nanomolar affinity ligands that retain a surprisingly high degree of specificity for the cognate pMHC target. Structural investigation of one such CDR2 mutant implicates shape complementarity of the mutant CDR2 contact interfaces as being a key determinant of the increased affinity. Our results suggest that manipulation of germ line CDR2 loops may provide a useful route to the production of high-affinity TCRs with therapeutic and diagnostic potential.
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(as it appears on PubMed at http://www.pubmed.gov), where 16600963 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16600963}}
==Disease==
==Disease==
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[[Category: T-cell receptor]]
[[Category: T-cell receptor]]
[[Category: Wild type sequence]]
[[Category: Wild type sequence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:43:01 2008''

Revision as of 08:43, 29 July 2008

Image:2f54.png

Template:STRUCTURE 2f54

Contents

Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity

Template:ABSTRACT PUBMED 16600963

Disease

Known disease associated with this structure: Hypoproteinemia, hypercatabolic OMIM:[109700]

About this Structure

2F54 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity., Dunn SM, Rizkallah PJ, Baston E, Mahon T, Cameron B, Moysey R, Gao F, Sami M, Boulter J, Li Y, Jakobsen BK, Protein Sci. 2006 Apr;15(4):710-21. PMID:16600963

Page seeded by OCA on Tue Jul 29 11:43:01 2008

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