This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.




2f56

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2f56.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2f56.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2f56| PDB=2f56 | SCENE= }}
{{STRUCTURE_2f56| PDB=2f56 | SCENE= }}
-
'''Barnase cross-linked with glutaraldehyde soaked in 6M urea'''
+
===Barnase cross-linked with glutaraldehyde soaked in 6M urea===
-
==Overview==
+
<!--
-
Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (&gt;or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect).
+
The line below this paragraph, {{ABSTRACT_PUBMED_16600702}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16600702 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16600702}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Thiourea]]
[[Category: Thiourea]]
[[Category: Urea]]
[[Category: Urea]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:28:25 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:40:00 2008''

Revision as of 11:40, 27 July 2008

Image:2f56.png

Template:STRUCTURE 2f56

Barnase cross-linked with glutaraldehyde soaked in 6M urea

Template:ABSTRACT PUBMED 16600702

About this Structure

2F56 is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations., Salem M, Mauguen Y, Prange T, Biochim Biophys Acta. 2006 May;1764(5):903-12. Epub 2006 Mar 20. PMID:16600702

Page seeded by OCA on Sun Jul 27 14:40:00 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2f56"
Personal tools