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2f9o

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[[Image:2f9o.gif|left|200px]]
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{{STRUCTURE_2f9o| PDB=2f9o | SCENE= }}
{{STRUCTURE_2f9o| PDB=2f9o | SCENE= }}
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'''Crystal Structure of the Recombinant Human Alpha I Tryptase Mutant D216G'''
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===Crystal Structure of the Recombinant Human Alpha I Tryptase Mutant D216G===
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==Overview==
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Tryptases alpha and beta are trypsin-like serine proteinases expressed in large amounts by mast cells. Beta-tryptase is a tetramer that has enzymatic activity, but requires heparin binding to maintain functional and structural stability, whereas alpha-tryptase has little, if any, enzymatic activity but is a stable tetramer in the absence of heparin. As shown previously, these differences can be mainly attributed to the different conformations of the 214-220 segment. Interestingly, the replacement of Asp216 by Gly, which is present in beta-tryptase, results in enzymatically active but less stable alpha-tryptase mutants. We have solved the crystal structures of both the single (D216G) and the double (K192Q/D216G) mutant forms of recombinant human alphaI-tryptase in complex with the peptide inhibitor leupeptin, as well as the structure of the non-inhibited single mutant. The inhibited mutants exhibited an open functional substrate binding site, while in the absence of an inhibitor, the open (beta-tryptase-like) and the closed (alpha-tryptase-like) conformations were present simultaneously. This shows that both forms are in a two-state equilibrium, which is influenced by the residues in the vicinity of the active site and by inhibitor/substrate binding. Novel insights regarding the observed stability differences as well as a potential proteolytic activity of wild-type alpha-tryptase, which may possess a cryptic active site, are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 16414069 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16414069}}
==About this Structure==
==About this Structure==
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[[Category: Serine proteinase]]
[[Category: Serine proteinase]]
[[Category: Trypsin-like]]
[[Category: Trypsin-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:38:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:01:31 2008''

Revision as of 18:01, 27 July 2008

Image:2f9o.png

Template:STRUCTURE 2f9o

Crystal Structure of the Recombinant Human Alpha I Tryptase Mutant D216G

Template:ABSTRACT PUBMED 16414069

About this Structure

2F9O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

X-ray structures of free and leupeptin-complexed human alphaI-tryptase mutants: indication for an alpha-->beta-tryptase transition., Rohr KB, Selwood T, Marquardt U, Huber R, Schechter NM, Bode W, Than ME, J Mol Biol. 2006 Mar 17;357(1):195-209. Epub 2005 Dec 28. PMID:16414069

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