2fc0

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[[Image:2fc0.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2fc0| PDB=2fc0 | SCENE= }}
{{STRUCTURE_2fc0| PDB=2fc0 | SCENE= }}
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'''WRN exonuclease, Mn dGMP complex'''
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===WRN exonuclease, Mn dGMP complex===
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==Overview==
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WRN is unique among the five human RecQ DNA helicases in having a functional exonuclease domain (WRN-exo) and being defective in the premature aging and cancer-related disorder Werner syndrome. Here, we characterize WRN-exo crystal structures, biochemical activity and participation in DNA end joining. Metal-ion complex structures, active site mutations and activity assays reveal a nuclease mechanism mediated by two metal ions. The DNA end-binding Ku70/80 complex specifically stimulates WRN-exo activity, and structure-based mutational inactivation of WRN-exo alters DNA end joining in human cells. We furthermore establish structural and biochemical similarities of WRN-exo to DnaQ-family replicative proofreading exonucleases, describing WRN-specific adaptations consistent with double-stranded DNA specificity and functionally important conformational changes. These results indicate WRN-exo is a human DnaQ family member and support DnaQ-like proofreading activities stimulated by Ku70/80, with implications for WRN functions in age-related pathologies and maintenance of genomic integrity.
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{{ABSTRACT_PUBMED_16622405}}
==About this Structure==
==About this Structure==
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[[Category: Werner syndrome]]
[[Category: Werner syndrome]]
[[Category: Wrn]]
[[Category: Wrn]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:38:19 2008''

Revision as of 21:38, 28 July 2008

Image:2fc0.png

Template:STRUCTURE 2fc0

WRN exonuclease, Mn dGMP complex

Template:ABSTRACT PUBMED 16622405

About this Structure

2FC0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing., Perry JJ, Yannone SM, Holden LG, Hitomi C, Asaithamby A, Han S, Cooper PK, Chen DJ, Tainer JA, Nat Struct Mol Biol. 2006 May;13(5):414-22. Epub 2006 Apr 23. PMID:16622405

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