This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2fik

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2fik.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2fik.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2fik| PDB=2fik | SCENE= }}
{{STRUCTURE_2fik| PDB=2fik | SCENE= }}
-
'''Structure of a microbial glycosphingolipid bound to mouse CD1d'''
+
===Structure of a microbial glycosphingolipid bound to mouse CD1d===
-
==Overview==
+
<!--
-
Natural killer T (NKT) cells provide an innate-type immune response upon T cell receptor interaction with CD1d-presented antigens. We demonstrate through equilibrium tetramer binding and antigen presentation assays with Valpha14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galacturonosyl ceramide (GalA-GSL) is a NKT cell agonist that is significantly weaker than alpha-galactosylceramide (alpha-GalCer), the most potent known NKT agonist. For GalA-GSL, a shorter fatty acyl chain, an absence of the 4-OH on the sphingosine tail and a 6'-COOH group on the galactose moiety account for its observed antigenic potency. We further determined the crystal structure of mCD1d in complex with GalA-GSL at 1.8-A resolution. The overall binding mode of GalA-GSL to mCD1d is similar to that of the short-chain alpha-GalCer ligand PBS-25, but its sphinganine chain is more deeply inserted into the F' pocket due to alternate hydrogen-bonding interactions between the sphinganine 3-OH with Asp-80. Subsequently, a slight lateral shift (&gt;1 A) of the galacturonosyl head group is noted at the CD1 surface compared with the galactose of alpha-GalCer. Because the relatively short C(14) fatty acid of GalA-GSL does not fully occupy the A' pocket, a spacer lipid is found that stabilizes this pocket. The lipid spacer was identified by GC/MS as a mixture of saturated and monounsaturated palmitic acid (C(16)). Comparison of available crystal structures of alpha-anomeric glycosphingolipids now sheds light on the structural basis of their differential antigenic potency and has led to the design and synthesis of NKT cell agonists with enhanced cell-based stimulatory activities compared with alpha-GalCer.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16537470}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16537470 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16537470}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Nkt cell]]
[[Category: Nkt cell]]
[[Category: Tcr]]
[[Category: Tcr]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:56:24 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:29:57 2008''

Revision as of 11:30, 29 July 2008

Image:2fik.png

Template:STRUCTURE 2fik

Structure of a microbial glycosphingolipid bound to mouse CD1d

Template:ABSTRACT PUBMED 16537470

About this Structure

2FIK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Design of natural killer T cell activators: structure and function of a microbial glycosphingolipid bound to mouse CD1d., Wu D, Zajonc DM, Fujio M, Sullivan BA, Kinjo Y, Kronenberg M, Wilson IA, Wong CH, Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):3972-7. Epub 2006 Mar 6. PMID:16537470

Page seeded by OCA on Tue Jul 29 14:29:57 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fik"
Personal tools