2fjs

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[[Image:2fjs.gif|left|200px]]
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{{Seed}}
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[[Image:2fjs.png|left|200px]]
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{{STRUCTURE_2fjs| PDB=2fjs | SCENE= }}
{{STRUCTURE_2fjs| PDB=2fjs | SCENE= }}
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'''Crystal Structure of Anaerobically Reduced Wild Type Nitrite Reductase from A. faecalis'''
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===Crystal Structure of Anaerobically Reduced Wild Type Nitrite Reductase from A. faecalis===
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==Overview==
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Copper-containing nitrite reductase harbors a type-1 and a type-2 Cu site. The former acts as the electron acceptor site of the enzyme, and the latter is the site of catalytic action. The effect of the methionine ligand on the reorganization energy of the type-1 site was explored by studying the electron-transfer kinetics between NiR (wild type (wt) and the variants Met150Gly and Met150Thr) with Fe(II)EDTA and Fe(II)HEDTA. The mutations increased the reorganization energy by 0.3 eV (30 kJ mol-1). A similar increase was found from pulse radiolysis experiments on the wt NIR and three variants (Met150Gly, Met150His, and Met150Thr). Binding of the nearby Met62 to the type-1 Cu site in Met150Gly (under influence of an allosteric effector) lowered the reorganization energy back to approximately the wt value. According to XRD data the structure of the reduced type-1 site in Met150Gly NiR in the presence of an allosteric effector is similar to that in the reduced wt NiR (solved to 1.85 A), compatible with the similarity in reorganization energy.
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(as it appears on PubMed at http://www.pubmed.gov), where 17227014 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17227014}}
==About this Structure==
==About this Structure==
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[[Category: Blue copper protein]]
[[Category: Blue copper protein]]
[[Category: Cupredoxin fold]]
[[Category: Cupredoxin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:58:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:41:34 2008''

Revision as of 10:41, 28 July 2008

Image:2fjs.png

Template:STRUCTURE 2fjs

Crystal Structure of Anaerobically Reduced Wild Type Nitrite Reductase from A. faecalis

Template:ABSTRACT PUBMED 17227014

About this Structure

2FJS is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

Effect of the methionine ligand on the reorganization energy of the type-1 copper site of nitrite reductase., Wijma HJ, MacPherson I, Farver O, Tocheva EI, Pecht I, Verbeet MP, Murphy ME, Canters GW, J Am Chem Soc. 2007 Jan 24;129(3):519-25. PMID:17227014

Page seeded by OCA on Mon Jul 28 13:41:34 2008

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