2c4i

From Proteopedia

Revision as of 10:11, 30 October 2007

CRYSTAL STRUCTURE OF ENGINEERED AVIDIN

Overview

Dual chain avidin (dcAvd) is an engineered avidin form, in which two, circularly permuted chicken avidin monomers are fused into one polypeptide, chain. DcAvd can theoretically form two different pseudotetrameric, quaternary assemblies because of symmetry at the monomer-monomer, interfaces. Here, our aim was to control the assembly of the quaternary, structure of dcAvd. We introduced the mutation I117C into one of the, circularly permuted domains of dcAvd and scanned residues along the 1-3, subunit interface of the other domain. Interestingly, V115H resulted in a, single, disulfide locked quaternary assembly of dcAvd, whereas I117H could, not guide the oligomerisation process even though it stabilised the, protein. The modified dcAvd forms were found to retain their, characteristic ... [(full description)]

About this Structure

2C4I is a [Single protein] structure of sequence from [Gallus gallus] with SO4 and BTN as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Controlling quaternary structure assembly: subunit interface engineering and crystal structure of dual chain avidin., Hytonen VP, Horha J, Airenne TT, Niskanen EA, Helttunen KJ, Johnson MS, Salminen TA, Kulomaa MS, Nordlund HR, J Mol Biol. 2006 Jun 23;359(5):1352-63. Epub 2006 May 6. PMID:16787776

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