2fnq

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{{STRUCTURE_2fnq| PDB=2fnq | SCENE= }}
{{STRUCTURE_2fnq| PDB=2fnq | SCENE= }}
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'''Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality'''
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===Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality===
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==Overview==
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Lipoxygenases (LOXs) catalyze the regio- and stereospecific dioxygenation of polyunsaturated membrane-embedded fatty acids. We report here the 3.2 A resolution structure of 8R-LOX from the Caribbean sea whip coral Plexaura homomalla, a LOX isozyme with calcium dependence and the uncommon R chiral stereospecificity. Structural and spectroscopic analyses demonstrated calcium binding in a C2-like membrane-binding domain, illuminating the function of similar amino acids in calcium-activated mammalian 5-LOX, the key enzyme in the pathway to the pro-inflammatory leukotrienes. Mutation of Ca(2+)-ligating amino acids in 8R-LOX resulted not only in a diminished capacity to bind membranes, as monitored by fluorescence resonance energy transfer, but also in an associated loss of Ca(2+)-regulated enzyme activity. Moreover, a structural basis for R chiral specificity is also revealed; creation of a small oxygen pocket next to Gly(428) (Ala in all S-LOX isozymes) promoted C-8 oxygenation with R chirality on the activated fatty acid substrate.
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{{ABSTRACT_PUBMED_16162493}}
==About this Structure==
==About this Structure==
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[[Category: Eicosanoid]]
[[Category: Eicosanoid]]
[[Category: Fatty acid]]
[[Category: Fatty acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:06:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:55:32 2008''

Revision as of 19:55, 28 July 2008

Image:2fnq.png

Template:STRUCTURE 2fnq

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality

Template:ABSTRACT PUBMED 16162493

About this Structure

2FNQ is a Single protein structure of sequence from Plexaura homomalla. This structure supersedes the now removed PDB entry 1zq4. Full crystallographic information is available from OCA.

Reference

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality., Oldham ML, Brash AR, Newcomer ME, J Biol Chem. 2005 Nov 25;280(47):39545-52. Epub 2005 Sep 14. PMID:16162493

Page seeded by OCA on Mon Jul 28 22:55:32 2008

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