2jox
From Proteopedia
(New page: 200px<br /> <applet load="2jox" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jox" /> '''Embryonic Neural Inducing Factor Churchill ...) |
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| - | [[Image:2jox. | + | [[Image:2jox.jpg|left|200px]]<br /><applet load="2jox" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed beta-Sheet and Zinc Binuclear Cluster'''<br /> | '''Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed beta-Sheet and Zinc Binuclear Cluster'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JOX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2JOX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:00:29 2008'' |
Revision as of 12:00, 23 January 2008
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Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed beta-Sheet and Zinc Binuclear Cluster
Overview
Churchill is a zinc-containing protein that is involved in neural, induction during embryogenesis. At the time of its discovery, it was, thought on the basis of sequence alignment to contain two zinc fingers of, the C(4) type. Further, binding of an N-terminal GST-Churchill fusion, protein to a particular DNA sequence was demonstrated by, immunoprecipitation selection assay, suggesting that Churchill may, function as a transcriptional regulator by sequence-specific DNA binding., We show by NMR solution structure determination that, far from containing, canonical C(4) zinc fingers, the protein contains three bound zinc ions in, novel coordination sites, including an unusual binuclear zinc cluster. The, secondary structure of Churchill is also unusual, consisting of a highly, solvent-exposed single-layer beta-sheet. Hydrogen-deuterium exchange and, backbone relaxation measurements reveal that Churchill is unusually, dynamic on a number of time scales, with the exception of regions, surrounding the zinc coordinating sites, which serve to stabilize the, otherwise unstructured N terminus and the single-layer beta-sheet. No, binding of Churchill to the previously identified DNA sequence could be, detected, and extensive searches using DNA sequence selection techniques, could find no other DNA sequence that was bound by Churchill. Since the, N-terminal amino acids of Churchill form part of the zinc-binding motif, the addition of a fusion protein at the N terminus causes loss of zinc and, unfolding of Churchill. This observation most likely explains the, published DNA-binding results, which would arise due to non-specific, interaction of the unfolded protein in the immunoprecipitation selection, assay. Since Churchill does not appear to bind DNA, we suggest that it may, function in embryogenesis as a protein-interaction factor.
About this Structure
2JOX is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Embryonic Neural Inducing Factor Churchill Is not a DNA-binding Zinc Finger Protein: Solution Structure Reveals a Solvent-exposed beta-Sheet and Zinc Binuclear Cluster., Lee BM, Buck-Koehntop BA, Martinez-Yamout MA, Dyson HJ, Wright PE, J Mol Biol. 2007 Aug 31;371(5):1274-89. Epub 2007 Jun 15. PMID:17610897
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