2ngr
From Proteopedia
(New page: 200px<br /> <applet load="2ngr" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ngr, resolution 1.9Å" /> '''TRANSITION STATE COM...) |
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caption="2ngr, resolution 1.9Å" /> | caption="2ngr, resolution 1.9Å" /> | ||
'''TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.'''<br /> | '''TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NGR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, GDP and AF3 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2NGR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GDP:'>GDP</scene> and <scene name='pdbligand=AF3:'>AF3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NGR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transition state]] | [[Category: transition state]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:38:37 2008'' |
Revision as of 15:38, 15 February 2008
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TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.
Overview
The Rho-related small GTP-binding protein Cdc42 has a low intrinsic GTPase, activity that is significantly enhanced by its specific GTPase-activating, protein, Cdc42GAP. In this report, we present the tertiary structure for, the aluminum fluoride-promoted complex between Cdc42 and a catalytically, active domain of Cdc42GAP as well as the complex between Cdc42 and the, catalytically compromised Cdc42GAP(R305A) mutant. These structures, which, mimic the transition state for the GTP hydrolytic reaction, show the, presence of an AIF3 molecule, as was seen for the corresponding, Ras-p120RasGAP complex, but in contrast to what has been reported for the, Rho-Cdc42GAP complex or for heterotrimeric G protein alpha subunits, where, AIF4- was observed. The Cdc42GAP stabilizes both the switch I and switch, II domains of Cdc42 and contributes a highly conserved arginine (Arg 305), to the active site. Comparison of the structures for the wild type and, mutant Cdc42GAP complexes provides important insights into the, GAP-catalyzed GTP hydrolytic reaction.
About this Structure
2NGR is a Protein complex structure of sequences from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP., Nassar N, Hoffman GR, Manor D, Clardy JC, Cerione RA, Nat Struct Biol. 1998 Dec;5(12):1047-52. PMID:9846874
Page seeded by OCA on Fri Feb 15 17:38:37 2008
Categories: Homo sapiens | Protein complex | Cerione, R. | Clardy, J. | Hoffman, G. | Nassar, N. | AF3 | GDP | MG | Alf3. | Cdc42 | G-protein | Gap | Transition state
