2fu5

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[[Image:2fu5.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2fu5| PDB=2fu5 | SCENE= }}
{{STRUCTURE_2fu5| PDB=2fu5 | SCENE= }}
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'''structure of Rab8 in complex with MSS4'''
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===structure of Rab8 in complex with MSS4===
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==Overview==
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Rab GTPases function as essential regulators of vesicle transport in eukaryotic cells. MSS4 was shown to stimulate nucleotide exchange on Rab proteins associated with the exocytic pathway and to have nucleotide-free-Rab chaperone activity. A detailed kinetic analysis of MSS4 interaction with Rab8 showed that MSS4 is a relatively slow exchange factor that forms a long-lived nucleotide-free complex with RabGTPase. In contrast to other characterized exchange factor-GTPase complexes, MSS4:Rab8 complex binds GTP faster than GDP, but still ca. 3 orders of magnitude more slowly than comparable complexes. The crystal structure of the nucleotide-free MSS4:Rab8 complex revealed that MSS4 binds to the Switch I and interswitch regions of Rab8, forming an intermolecular beta-sheet. Complex formation results in dramatic structural changes of the Rab8 molecule, leading to unfolding of the nucleotide-binding site and surrounding structural elements, facilitating nucleotide release and slowing its rebinding. Coupling of nucleotide exchange activity to a cycle of GTPase unfolding and refolding represents a novel nucleotide exchange mechanism.
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(as it appears on PubMed at http://www.pubmed.gov), where 16541104 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16541104}}
==About this Structure==
==About this Structure==
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[[Category: Mss4:rab8 protein complex]]
[[Category: Mss4:rab8 protein complex]]
[[Category: Nucleotide exchange via unfolding of nucleotide binding region]]
[[Category: Nucleotide exchange via unfolding of nucleotide binding region]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:18:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:08:35 2008''

Revision as of 20:08, 28 July 2008

Image:2fu5.png

Template:STRUCTURE 2fu5

structure of Rab8 in complex with MSS4

Template:ABSTRACT PUBMED 16541104

About this Structure

2FU5 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

Reference

Nucleotide exchange via local protein unfolding--structure of Rab8 in complex with MSS4., Itzen A, Pylypenko O, Goody RS, Alexandrov K, Rak A, EMBO J. 2006 Apr 5;25(7):1445-55. Epub 2006 Mar 16. PMID:16541104

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