2fxp
From Proteopedia
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'''Solution Structure of the SARS-Coronavirus HR2 Domain''' | '''Solution Structure of the SARS-Coronavirus HR2 Domain''' | ||
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| + | ==Overview== | ||
| + | The envelope glycoprotein, termed the spike protein, of severe acute respiratory syndrome coronavirus (SARS-CoV) is known to mediate viral entry. Similar to other class 1 viral fusion proteins, the heptad repeat regions of SARS-CoV spike are thought to undergo conformational changes from a prefusion form to a subsequent post-fusion form that enables fusion of the viral and host membranes. Recently, the structure of a post-fusion form of SARS-CoV spike, which consists of isolated domains of heptad repeats 1 and 2 (HR1 and HR2), has been determined by x-ray crystallography. To date there is no structural information for the prefusion conformations of SARS-CoV HR1 and HR2. In this work we present the NMR structure of the HR2 domain (residues 1141-1193) from SARS-CoV (termed S2-HR2) in the presence of the co-solvent trifluoroethanol. We find that in the absence of HR1, S2-HR2 forms a coiled coil symmetric trimer with a complex molecular mass of 18 kDa. The S2-HR2 structure, which is the first example of the prefusion form of coronavirus envelope, supports the current model of viral membrane fusion and gives insight into the design of structure-based antagonists of SARS. | ||
==About this Structure== | ==About this Structure== | ||
2FXP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FXP OCA]. | 2FXP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FXP OCA]. | ||
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| + | ==Reference== | ||
| + | Solution structure of the severe acute respiratory syndrome-coronavirus heptad repeat 2 domain in the prefusion state., Hakansson-McReynolds S, Jiang S, Rong L, Caffrey M, J Biol Chem. 2006 Apr 28;281(17):11965-71. Epub 2006 Feb 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16507566 16507566] | ||
[[Category: Human sars coronavirus]] | [[Category: Human sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Prefusion intermediate]] | [[Category: Prefusion intermediate]] | ||
[[Category: Trimer]] | [[Category: Trimer]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | [[Category: Virus/viral protein complex]] |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:22:31 2008'' | ||
Revision as of 19:22, 22 May 2008
Solution Structure of the SARS-Coronavirus HR2 Domain
Overview
The envelope glycoprotein, termed the spike protein, of severe acute respiratory syndrome coronavirus (SARS-CoV) is known to mediate viral entry. Similar to other class 1 viral fusion proteins, the heptad repeat regions of SARS-CoV spike are thought to undergo conformational changes from a prefusion form to a subsequent post-fusion form that enables fusion of the viral and host membranes. Recently, the structure of a post-fusion form of SARS-CoV spike, which consists of isolated domains of heptad repeats 1 and 2 (HR1 and HR2), has been determined by x-ray crystallography. To date there is no structural information for the prefusion conformations of SARS-CoV HR1 and HR2. In this work we present the NMR structure of the HR2 domain (residues 1141-1193) from SARS-CoV (termed S2-HR2) in the presence of the co-solvent trifluoroethanol. We find that in the absence of HR1, S2-HR2 forms a coiled coil symmetric trimer with a complex molecular mass of 18 kDa. The S2-HR2 structure, which is the first example of the prefusion form of coronavirus envelope, supports the current model of viral membrane fusion and gives insight into the design of structure-based antagonists of SARS.
About this Structure
2FXP is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.
Reference
Solution structure of the severe acute respiratory syndrome-coronavirus heptad repeat 2 domain in the prefusion state., Hakansson-McReynolds S, Jiang S, Rong L, Caffrey M, J Biol Chem. 2006 Apr 28;281(17):11965-71. Epub 2006 Feb 28. PMID:16507566 Page seeded by OCA on Thu May 22 22:22:31 2008
