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2g0f

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{{STRUCTURE_2g0f| PDB=2g0f | SCENE= }}
{{STRUCTURE_2g0f| PDB=2g0f | SCENE= }}
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'''Crystal Structure of P144A mutant of E.coli CcmG protein'''
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===Crystal Structure of P144A mutant of E.coli CcmG protein===
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==Overview==
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CcmG, also designated DsbE, functions as a periplasmic protein thiol:disulfide oxidoreductase and is required for cytochrome c maturation. Here we report the crystal structures of Escherichia coli CcmG and its two mutants, P144A and the N-terminal fifty seven-residue deletion mutant, and two additional deletion mutants were studied by circular dichroism. Structural comparison of E. coli CcmG with its deletion mutants reveals that the N-terminal beta-sheet is essential for maintaining the folding topology and consequently maintaining the active-site structure of CcmG. Pro144 and Glu145 are key residues of the fingerprint region of CcmG. Pro144 is in cis-configuration, and it makes van der Waals interactions with the active-site disulfide Cys80-Cys83 and forms a C--H...O hydrogen bond with Thr82, helping stabilize the active-site structure. Glu145 forms a salt-bridge and hydrogen-bond network with other residues of the fingerprint region and with Arg158, further stabilizing the active-site structure. The cis-configuration of Pro144 makes the backbone nitrogen and oxygen of Ala143 exposed to solvent, favorable for interacting with binding partners. The key role of cis-Pro144 is verified by the P144A mutant, which contains trans-Ala144 and displays redox property changes. Structural comparison of E. coli CcmG with the recently reported structure of CcmG in complex with the N-terminal domain of DsbD reveals that Tyr141 undergoes conformational changes upon binding DsbD. A cis-proline located at the N-terminus of the first beta-strand of the betabetaalpha motif of the thioredoxin-like domain is a conserved structural feature of the thioredoxin superfamily.
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{{ABSTRACT_PUBMED_17019698}}
==About this Structure==
==About this Structure==
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[[Category: E coli ccmg]]
[[Category: E coli ccmg]]
[[Category: P144a mutant]]
[[Category: P144a mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:57:41 2008''

Revision as of 17:57, 27 July 2008

Image:2g0f.png

Template:STRUCTURE 2g0f

Crystal Structure of P144A mutant of E.coli CcmG protein

Template:ABSTRACT PUBMED 17019698

About this Structure

2G0F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of E. coli CcmG and its mutants reveal key roles of the N-terminal beta-sheet and the fingerprint region., Ouyang N, Gao YG, Hu HY, Xia ZX, Proteins. 2006 Dec 1;65(4):1021-31. PMID:17019698

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