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| | {{STRUCTURE_2g3k| PDB=2g3k | SCENE= }} | | {{STRUCTURE_2g3k| PDB=2g3k | SCENE= }} |
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| - | '''Crystal structure of the C-terminal domain of Vps28'''
| + | ===Crystal structure of the C-terminal domain of Vps28=== |
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| - | ==Overview==
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| - | The endosomal sorting complex I required for transport (ESCRT-I) is composed of the three subunits Vps23/Tsg101, Vps28 and Vps37. ESCRT-I is recruited to cellular membranes during multivesicular endosome biogenesis and by enveloped viruses such as HIV-1 to mediate budding from the cell. Here, we describe the crystal structure of a conserved C-terminal domain from Sacharomyces cerevisiae Vps28 (Vps28-CTD) at 3.05 A resolution which folds independently into a four-helical bundle structure. Co-expression experiments of Vps28-CTD, Vps23 and Vps37 suggest that Vps28-CTD does not directly participate in ESCRT-I assembly and may thus act as an adaptor module for downstream interaction partners. We show through mutagenesis studies that Vps28-CTD employs its strictly conserved surface in the interaction with the ESCRT-III factor Vps20. Furthermore, we present evidence that Vps28-CTD is sufficient to rescue an equine infectious anaemia virus (EIAV) Gag late domain deletion. Vps28-CTD mutations abolishing Vps20 interaction in vitro also prevent the rescue of the EIAV Gag late domain mutant consistent with a potential direct Vps28-ESCRT-III Vps20 recruitment. Therefore, the physiological relevant EIAV Gag-Alix interaction can be functionally replaced by a Gag-Vps28-CTD fusion. Because both Alix and Vps28-CTD can directly recruit ESCRT-III proteins, ESCRT-III assembly coupled to Vps4 action may therefore constitute the minimal budding machinery for EIAV release. | + | The line below this paragraph, {{ABSTRACT_PUBMED_16749904}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16749904 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16749904}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Weissenhorn, W.]] | | [[Category: Weissenhorn, W.]] |
| | [[Category: 4 helix bundle]] | | [[Category: 4 helix bundle]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:39:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:14:51 2008'' |
Revision as of 06:14, 29 July 2008
Template:STRUCTURE 2g3k
Crystal structure of the C-terminal domain of Vps28
Template:ABSTRACT PUBMED 16749904
About this Structure
2G3K is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment., Pineda-Molina E, Belrhali H, Piefer AJ, Akula I, Bates P, Weissenhorn W, Traffic. 2006 Aug;7(8):1007-16. Epub 2006 Jun 2. PMID:16749904
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