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2g4b

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{{STRUCTURE_2g4b| PDB=2g4b | SCENE= }}
{{STRUCTURE_2g4b| PDB=2g4b | SCENE= }}
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'''Structure of U2AF65 variant with polyuridine tract'''
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===Structure of U2AF65 variant with polyuridine tract===
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==Overview==
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The essential pre-mRNA splicing factor, U2AF(65), guides the early stages of splice site choice by recognizing a polypyrimidine (Py) tract consensus sequence near the 3' splice site. Since Py tracts are relatively poorly conserved in higher eukaryotes, U2AF(65) is faced with the problem of specifying uridine-rich sequences, yet tolerating a variety of nucleotide substitutions found in natural Py tracts. To better understand these apparently contradictory RNA binding characteristics, the X-ray structure of the U2AF(65) RNA binding domain bound to a Py tract composed of seven uridines has been determined at 2.5 A resolution. Specific hydrogen bonds between U2AF(65) and the uracil bases provide an explanation for polyuridine recognition. Flexible side chains and bound water molecules form the majority of the base contacts and potentially could rearrange when the U2AF(65) structure adapts to different Py tract sequences. The energetic importance of conserved residues for Py tract binding is established by analysis of site-directed mutant U2AF(65) proteins using surface plasmon resonance.
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The line below this paragraph, {{ABSTRACT_PUBMED_16818232}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16818232 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16818232}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65., Sickmier EA, Frato KE, Shen H, Paranawithana SR, Green MR, Kielkopf CL, Mol Cell. 2006 Jul 7;23(1):49-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16818232 16818232]
Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65., Sickmier EA, Frato KE, Shen H, Paranawithana SR, Green MR, Kielkopf CL, Mol Cell. 2006 Jul 7;23(1):49-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16818232 16818232]
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Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering., Sickmier EA, Frato KE, Kielkopf CL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt, 5):457-9. Epub 2006 Apr 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16682775 16682775]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]
[[Category: Rna splicing factor]]
[[Category: Rna splicing factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:40:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:23:21 2008''

Revision as of 12:23, 29 July 2008

Image:2g4b.png

Template:STRUCTURE 2g4b

Structure of U2AF65 variant with polyuridine tract

Template:ABSTRACT PUBMED 16818232

About this Structure

2G4B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65., Sickmier EA, Frato KE, Shen H, Paranawithana SR, Green MR, Kielkopf CL, Mol Cell. 2006 Jul 7;23(1):49-59. PMID:16818232

Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering., Sickmier EA, Frato KE, Kielkopf CL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt, 5):457-9. Epub 2006 Apr 12. PMID:16682775

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