This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2g75

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2g75.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2g75.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2g75| PDB=2g75 | SCENE= }}
{{STRUCTURE_2g75| PDB=2g75 | SCENE= }}
-
'''Crystal Structure of anti-SARS m396 Antibody'''
+
===Crystal Structure of anti-SARS m396 Antibody===
-
==Overview==
+
<!--
-
The severe acute respiratory syndrome coronavirus (SARS-CoV, or SCV), which caused a world-wide epidemic in 2002 and 2003, binds to a receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) of its envelope (spike, S) glycoprotein. The RBD is very immunogenic; it is a major SCV neutralization determinant and can elicit potent neutralizing antibodies capable of out-competing ACE2. However, the structural basis of RBD immunogenicity, RBD-mediated neutralization, and the role of RBD in entry steps following its binding to ACE2 have not been elucidated. By mimicking immune responses with the use of RBD as an antigen to screen a large human antibody library derived from healthy volunteers, we identified a novel potent cross-reactive SCV-neutralizing monoclonal antibody, m396, which competes with ACE2 for binding to RBD, and determined the crystal structure of the RBD-antibody complex at 2.3-A resolution. The antibody-bound RBD structure is completely defined, revealing two previously unresolved segments (residues 376-381 and 503-512) and a new disulfide bond (between residues 378 and 511). Interestingly, the overall structure of the m396-bound RBD is not significantly different from that of the ACE2-bound RBD. The antibody epitope is dominated by a 10-residue-long protruding beta6-beta7 loop with two putative ACE2-binding hotspot residues (Ile-489 and Tyr-491). These results provide a structural rationale for the function of a major determinant of SCV immunogenicity and neutralization, the development of SCV therapeutics based on the antibody paratope and epitope, and a retrovaccinology approach for the design of anti-SCV vaccines. The available structural information indicates that the SCV entry may not be mediated by ACE2-induced conformational changes in the RBD but may involve other conformational changes or/and yet to be identified coreceptors.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16597622}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16597622 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16597622}}
==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Sar]]
[[Category: Sar]]
[[Category: Vaccine]]
[[Category: Vaccine]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:46:05 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:15:43 2008''

Revision as of 06:15, 28 July 2008

Image:2g75.png

Template:STRUCTURE 2g75

Crystal Structure of anti-SARS m396 Antibody

Template:ABSTRACT PUBMED 16597622

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure of severe acute respiratory syndrome coronavirus receptor-binding domain complexed with neutralizing antibody., Prabakaran P, Gan J, Feng Y, Zhu Z, Choudhry V, Xiao X, Ji X, Dimitrov DS, J Biol Chem. 2006 Jun 9;281(23):15829-36. Epub 2006 Apr 5. PMID:16597622

Page seeded by OCA on Mon Jul 28 09:15:43 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2g75"
Personal tools