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2nzi
From Proteopedia
(New page: 200px<br /> <applet load="2nzi" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nzi, resolution 2.9Å" /> '''Crystal structure of...) |
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| - | [[Image:2nzi.gif|left|200px]]<br /> | + | [[Image:2nzi.gif|left|200px]]<br /><applet load="2nzi" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2nzi" size=" | + | |
caption="2nzi, resolution 2.9Å" /> | caption="2nzi, resolution 2.9Å" /> | ||
'''Crystal structure of domains A168-A170 from titin'''<br /> | '''Crystal structure of domains A168-A170 from titin'''<br /> | ||
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==Overview== | ==Overview== | ||
Titin forms an intrasarcomeric filament system in vertebrate striated, muscle, which has elastic and signaling properties and is thereby central, to mechanotransduction. Near its C-terminus and directly preceding a, kinase domain, titin contains a conserved pattern of Ig and FnIII modules, (Ig(A168)-Ig(A169)-FnIII(A170), hereby A168-A170) that recruits the E3, ubiquitin-ligase MuRF-1 to the filament. This interaction is thought to, regulate myofibril turnover and the trophic state of muscle. We have, elucidated the crystal structure of A168-A170, characterized MuRF-1, variants by cluster of differentiation (CD) and SEC-MALS, and studied the, interaction of both components by isothermal calorimetry, SPOTS blots, and, pull-down assays. This has led to the identification of the molecular, determinants of the binding. A168-A170 shows an extended, rigid, architecture, which is characterized by a shallow surface groove that, spans its full length and a distinct loop protrusion in its middle point., In MuRF-1, a C-terminal helical domain is sufficient to bind A168-A170, with high affinity. This helical region predictably docks into the surface, groove of A168-A170. Furthermore, pull-down assays demonstrate that the, loop protrusion in A168-A170 is a key mediator of MuRF-1 recognition. Our, findings indicate that this region of titin could serve as a target to, attempt therapeutic inhibition of MuRF-1-mediated muscle turnover, where, binding of small molecules to its distinctive structural features could, block MuRF-1 access.--Mrosek, M., Labeit, D., Witt, S., Heerklotz, H., von, Castelmur, E., Labeit, S., Mayans, O. Molecular determinants for the, recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin. | Titin forms an intrasarcomeric filament system in vertebrate striated, muscle, which has elastic and signaling properties and is thereby central, to mechanotransduction. Near its C-terminus and directly preceding a, kinase domain, titin contains a conserved pattern of Ig and FnIII modules, (Ig(A168)-Ig(A169)-FnIII(A170), hereby A168-A170) that recruits the E3, ubiquitin-ligase MuRF-1 to the filament. This interaction is thought to, regulate myofibril turnover and the trophic state of muscle. We have, elucidated the crystal structure of A168-A170, characterized MuRF-1, variants by cluster of differentiation (CD) and SEC-MALS, and studied the, interaction of both components by isothermal calorimetry, SPOTS blots, and, pull-down assays. This has led to the identification of the molecular, determinants of the binding. A168-A170 shows an extended, rigid, architecture, which is characterized by a shallow surface groove that, spans its full length and a distinct loop protrusion in its middle point., In MuRF-1, a C-terminal helical domain is sufficient to bind A168-A170, with high affinity. This helical region predictably docks into the surface, groove of A168-A170. Furthermore, pull-down assays demonstrate that the, loop protrusion in A168-A170 is a key mediator of MuRF-1 recognition. Our, findings indicate that this region of titin could serve as a target to, attempt therapeutic inhibition of MuRF-1-mediated muscle turnover, where, binding of small molecules to its distinctive structural features could, block MuRF-1 access.--Mrosek, M., Labeit, D., Witt, S., Heerklotz, H., von, Castelmur, E., Labeit, S., Mayans, O. Molecular determinants for the, recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin. | ||
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| - | ==Disease== | ||
| - | Known diseases associated with this structure: Cardiomyopathy, dilated, 1G OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=188840 188840]], Cardiomyopathy, familial hypertrophic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=188840 188840]], Muscular dystrophy, limb-girdle, type 2J OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=188840 188840]], Myopathy, proximal, with early respiratory muscle involvement OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=188840 188840]], Tibial muscular dystrophy, tardive OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=188840 188840]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 2NZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http:// | + | 2NZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ig-domain]] | [[Category: ig-domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:03 2008'' |
Revision as of 13:15, 23 January 2008
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Crystal structure of domains A168-A170 from titin
Overview
Titin forms an intrasarcomeric filament system in vertebrate striated, muscle, which has elastic and signaling properties and is thereby central, to mechanotransduction. Near its C-terminus and directly preceding a, kinase domain, titin contains a conserved pattern of Ig and FnIII modules, (Ig(A168)-Ig(A169)-FnIII(A170), hereby A168-A170) that recruits the E3, ubiquitin-ligase MuRF-1 to the filament. This interaction is thought to, regulate myofibril turnover and the trophic state of muscle. We have, elucidated the crystal structure of A168-A170, characterized MuRF-1, variants by cluster of differentiation (CD) and SEC-MALS, and studied the, interaction of both components by isothermal calorimetry, SPOTS blots, and, pull-down assays. This has led to the identification of the molecular, determinants of the binding. A168-A170 shows an extended, rigid, architecture, which is characterized by a shallow surface groove that, spans its full length and a distinct loop protrusion in its middle point., In MuRF-1, a C-terminal helical domain is sufficient to bind A168-A170, with high affinity. This helical region predictably docks into the surface, groove of A168-A170. Furthermore, pull-down assays demonstrate that the, loop protrusion in A168-A170 is a key mediator of MuRF-1 recognition. Our, findings indicate that this region of titin could serve as a target to, attempt therapeutic inhibition of MuRF-1-mediated muscle turnover, where, binding of small molecules to its distinctive structural features could, block MuRF-1 access.--Mrosek, M., Labeit, D., Witt, S., Heerklotz, H., von, Castelmur, E., Labeit, S., Mayans, O. Molecular determinants for the, recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin.
About this Structure
2NZI is a Single protein structure of sequence from Homo sapiens. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
Molecular determinants for the recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin., Mrosek M, Labeit D, Witt S, Heerklotz H, von Castelmur E, Labeit S, Mayans O, FASEB J. 2007 Jan 10;. PMID:17215480
Page seeded by OCA on Wed Jan 23 15:15:03 2008
