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2gah

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{{STRUCTURE_2gah| PDB=2gah | SCENE= }}
{{STRUCTURE_2gah| PDB=2gah | SCENE= }}
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'''Heterotetrameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution'''
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===Heterotetrameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution===
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==Overview==
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The crystal structure of heterotetrameric sarcosine oxidase (TSOX) from Pseudomonas maltophilia has been determined at 1.85 A resolution. TSOX contains three coenzymes (FAD, FMN and NAD+), four different subunits (alpha, 103 kDa; beta, 44 kDa; gamma, 21 kDa; delta, 11 kDa) and catalyzes the oxidation of sarcosine (N-methylglycine) to yield hydrogen peroxide, glycine and formaldehyde. In the presence of tetrahydrofolate, the oxidation of sarcosine is coupled to the formation of 5,10-methylenetetrahydrofolate. The NAD+ and putative folate binding sites are located in the alpha-subunit. The FAD binding site is in the beta-subunit. FMN is bound at the interface of the alpha and beta-subunits. The FAD and FMN rings are separated by a short segment of the beta-subunit with the closest atoms located 7.4 A apart. Sulfite, an inhibitor of oxygen reduction, is bound at the FMN site. 2-Furoate, a competitive inhibitor with respect to sarcosine, is bound at the FAD site. The sarcosine dehydrogenase and 5,10-methylenetetrahydrofolate synthase sites are 35 A apart but connected by a large internal cavity (approximately 10,000 A3). An unexpected zinc ion, coordinated by three cysteine and one histidine side-chains, is bound to the delta-subunit. The N-terminal half of the alpha subunit of TSOX (alphaA) is closely similar to the FAD-binding domain of glutathione reductase but with NAD+ replacing FAD. The C-terminal half of the alpha subunit of TSOX (alphaB) is similar to the C-terminal half of dimethylglycine oxidase and the T-protein of the glycine cleavage system, proteins that bind tetrahydrofolate. The beta-subunit of TSOX is very similar to monomeric sarcosine oxidase. The gamma-subunit is similar to the C-terminal sub-domain of alpha-TSOX. The delta-subunit shows little similarity with any PDB entry. The alphaA domain/beta-subunit sub-structure of TSOX closely resembles the alphabeta dimer of L-proline dehydrogenase, a heteroctameric protein (alphabeta)4 that shows highest overall similarity to TSOX.
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{{ABSTRACT_PUBMED_16820168}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Heterotetrameric sarcosine oxidase: structure of a diflavin metalloenzyme at 1.85 A resolution., Chen ZW, Hassan-Abdulah A, Zhao G, Jorns MS, Mathews FS, J Mol Biol. 2006 Jul 28;360(5):1000-18. Epub 2006 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16820168 16820168]
Heterotetrameric sarcosine oxidase: structure of a diflavin metalloenzyme at 1.85 A resolution., Chen ZW, Hassan-Abdulah A, Zhao G, Jorns MS, Mathews FS, J Mol Biol. 2006 Jul 28;360(5):1000-18. Epub 2006 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16820168 16820168]
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Cloning, expression and crystallization of heterotetrameric sarcosine oxidase from Pseudomonas maltophilia., Hassan-Abdallah A, Zhao G, Eschenbrenner M, Chen ZW, Mathews FS, Jorns MS, Protein Expr Purif. 2005 Sep;43(1):33-43. Epub 2005 Apr 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15922624 15922624]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Stenotrophomonas maltophilia]]
[[Category: Stenotrophomonas maltophilia]]
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[[Category: Folate-methylating enzyme]]
[[Category: Folate-methylating enzyme]]
[[Category: Sarcosine oxidase]]
[[Category: Sarcosine oxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:06:45 2008''

Revision as of 02:06, 29 July 2008

Image:2gah.png

Template:STRUCTURE 2gah

Heterotetrameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution

Template:ABSTRACT PUBMED 16820168

About this Structure

2GAH is a Protein complex structure of sequences from Stenotrophomonas maltophilia. Full crystallographic information is available from OCA.

Reference

Heterotetrameric sarcosine oxidase: structure of a diflavin metalloenzyme at 1.85 A resolution., Chen ZW, Hassan-Abdulah A, Zhao G, Jorns MS, Mathews FS, J Mol Biol. 2006 Jul 28;360(5):1000-18. Epub 2006 Jun 15. PMID:16820168

Cloning, expression and crystallization of heterotetrameric sarcosine oxidase from Pseudomonas maltophilia., Hassan-Abdallah A, Zhao G, Eschenbrenner M, Chen ZW, Mathews FS, Jorns MS, Protein Expr Purif. 2005 Sep;43(1):33-43. Epub 2005 Apr 13. PMID:15922624

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