2gd7

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{{STRUCTURE_2gd7| PDB=2gd7 | SCENE= }}
{{STRUCTURE_2gd7| PDB=2gd7 | SCENE= }}
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'''The Structure of the Cyclin T-binding domain of Hexim1 reveals the molecular basis for regulation of transcription elongation'''
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===The Structure of the Cyclin T-binding domain of Hexim1 reveals the molecular basis for regulation of transcription elongation===
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==Overview==
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Hexim1 is a cellular protein that associates with the positive transcription elongation factor b (P-TEFb) to regulate RNA polymerase II elongation of nascent mRNA transcripts. It directly binds to Cyclin T1 of P-TEFb and inhibits the kinase activity of Cdk9, leading to an arrest of transcription elongation. Here, we report the solution structure of the Cyclin T binding domain (TBD) of Hexim1 that forms a parallel coiled-coil homodimer composed of two segments and a preceding alpha helix that folds back onto the first coiled-coil unit. NMR titration, fluorescence, and immunoprecipitation experiments revealed the binding interface to Cyclin T1, which covers a large surface on the first coiled-coil segment. Electrostatic interactions between an acidic patch on Hexim1 and positively charged residues of Cyclin T1 drive the complex formation that is confirmed by mutagenesis data on Hexim1 mediated transcription regulation in cells. Thus, our studies provide structural insights how Hexim1 recognizes the Cyclin T1 subunit of P-TEFb, which is a key step toward the regulation of transcription elongation.
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{{ABSTRACT_PUBMED_17724342}}
==About this Structure==
==About this Structure==
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2GD7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GD7 OCA].
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2GD7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GD7 OCA].
==Reference==
==Reference==
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[[Category: Transcription elongation]]
[[Category: Transcription elongation]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:58:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:34:51 2008''

Revision as of 08:34, 28 July 2008

Image:2gd7.png

Template:STRUCTURE 2gd7

The Structure of the Cyclin T-binding domain of Hexim1 reveals the molecular basis for regulation of transcription elongation

Template:ABSTRACT PUBMED 17724342

About this Structure

2GD7 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure of the Cyclin T binding domain of Hexim1 and molecular basis for its recognition of P-TEFb., Dames SA, Schonichen A, Schulte A, Barboric M, Peterlin BM, Grzesiek S, Geyer M, Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14312-7. Epub 2007 Aug 27. PMID:17724342

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