2gdn
From Proteopedia
| Line 28: | Line 28: | ||
[[Category: Wang, F.]] | [[Category: Wang, F.]] | ||
[[Category: Beta lactamase]] | [[Category: Beta lactamase]] | ||
| + | [[Category: Hydrolase]] | ||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Protein structure initiative]] | [[Category: Protein structure initiative]] | ||
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[[Category: Tb structural genomics consortium]] | [[Category: Tb structural genomics consortium]] | ||
[[Category: Tbsgc]] | [[Category: Tbsgc]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 5 10:02:38 2008'' |
Revision as of 07:02, 5 June 2008
Crystal structure of the Mycobacterium tuberculosis beta-lactamase
Overview
beta-Lactam antibiotics are extremely effective in disrupting the synthesis of the bacterial cell wall in both gram-positive and gram-negative bacteria. However, they are ineffective against Mycobacterium tuberculosis, due to the production of a beta-lactamase enzyme encoded on the chromosome of M. tuberculosis that degrades these antibiotics. Indeed, recent studies have demonstrated that deletion of the blaC gene, the only gene encoding a beta-lactamase in M. tuberculosis, or inhibition of the encoded enzyme resulted in significantly increased sensitivity to beta-lactam antibiotics. In this paper we present a biochemical and structural characterization of M. tuberculosis BlaC. Recombinant BlaC shows a broad range of specificity with almost equal penicillinase and cepholothinase activity. While clavulanate is a mechanism-based inhibitor to class A beta-lactamase with high potency (typically K(i) < 0.1 microM), it is a relatively poor inhibitor of the M. tuberculosis BlaC (K(i) = 2.4 microM). The crystal structure of the enzyme, determined at a resolution of 1.7 A, shows that the overall fold of the M. tuberculosis enzyme is similar to other class A beta-lactamases. There are, however, several distinct features of the active site, such as the amino acid substitutions N132G, R164A, R244A, and R276E, that explain the broad specificity of the enzyme, relatively low penicillinase activity, and resistance to clavulanate.
About this Structure
2GDN is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
Crystal structure and activity studies of the Mycobacterium tuberculosis beta-lactamase reveal its critical role in resistance to beta-lactam antibiotics., Wang F, Cassidy C, Sacchettini JC, Antimicrob Agents Chemother. 2006 Aug;50(8):2762-71. PMID:16870770 Page seeded by OCA on Thu Jun 5 10:02:38 2008
