2gdu

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{{STRUCTURE_2gdu| PDB=2gdu | SCENE= }}
{{STRUCTURE_2gdu| PDB=2gdu | SCENE= }}
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'''E232Q mutant of sucrose phosphorylase from BIFIDOBACTERIUM ADOLESCENTIS in complex with sucrose'''
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===E232Q mutant of sucrose phosphorylase from BIFIDOBACTERIUM ADOLESCENTIS in complex with sucrose===
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==Overview==
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The reaction mechanism of sucrose phosphorylase from Bifidobacterium adolescentis (BiSP) was studied by site-directed mutagenesis and x-ray crystallography. An inactive mutant of BiSP (E232Q) was co-crystallized with sucrose. The structure revealed a substrate-binding mode comparable with that seen in other related sucrose-acting enzymes. Wild-type BiSP was also crystallized in the presence of sucrose. In the dimeric structure, a covalent glucosyl intermediate was formed in one molecule of the BiSP dimer, and after hydrolysis of the glucosyl intermediate, a beta-D-glucose product complex was formed in the other molecule. Although the overall structure of the BiSP-glucosyl intermediate complex is similar to that of the BiSP(E232Q)-sucrose complex, the glucose complex discloses major differences in loop conformations. Two loops (residues 336-344 and 132-137) in the proximity of the active site move up to 16 and 4 A, respectively. On the basis of these findings, we have suggested a reaction cycle that takes into account the large movements in the active-site entrance loops.
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(as it appears on PubMed at http://www.pubmed.gov), where 16990265 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16990265}}
==About this Structure==
==About this Structure==
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[[Category: Dimer]]
[[Category: Dimer]]
[[Category: Glycoside hydrolase]]
[[Category: Glycoside hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:59:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:55:43 2008''

Revision as of 04:55, 29 July 2008

Image:2gdu.png

Template:STRUCTURE 2gdu

E232Q mutant of sucrose phosphorylase from BIFIDOBACTERIUM ADOLESCENTIS in complex with sucrose

Template:ABSTRACT PUBMED 16990265

About this Structure

2GDU is a Single protein structure of sequence from Bifidobacterium adolescentis. Full crystallographic information is available from OCA.

Reference

Structural rearrangements of sucrose phosphorylase from Bifidobacterium adolescentis during sucrose conversion., Mirza O, Skov LK, Sprogoe D, van den Broek LA, Beldman G, Kastrup JS, Gajhede M, J Biol Chem. 2006 Nov 17;281(46):35576-84. Epub 2006 Sep 21. PMID:16990265

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