We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2ghq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ghq.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2ghq.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ghq| PDB=2ghq | SCENE= }}
{{STRUCTURE_2ghq| PDB=2ghq | SCENE= }}
-
'''CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II'''
+
===CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II===
-
==Overview==
+
<!--
-
Phosphorylation and dephosphorylation of the C-terminal domain (CTD) of RNA polymerase II (Pol II) represent a critical regulatory checkpoint for transcription. Transcription initiation requires Fcp1/Scp1-mediated dephosphorylation of phospho-CTD. Fcp1 and Scp1 belong to a family of Mg2+ -dependent phosphoserine (P.Ser)/phosphothreonine (P.Thr)-specific phosphatases. We recently showed that Scp1 is an evolutionarily conserved regulator of neuronal gene silencing. Here, we present the X-ray crystal structures of a dominant-negative form of human Scp1 (D96N mutant) bound to mono- and diphosphorylated peptides encompassing the CTD heptad repeat (Y1S2P3T4S5P6S7). Moreover, kinetic and thermodynamic analyses of Scp1-phospho-CTD peptide complexes support the structures determined. This combined structure-function analysis discloses the residues in Scp1 involved in CTD binding and its preferential dephosphorylation of P.Ser5 of the CTD heptad repeat. Moreover, these results provide a template for the design of specific inhibitors of Scp1 for the study of neuronal stem cell development.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17157258}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17157258 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17157258}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Had superfamily]]
[[Category: Had superfamily]]
[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:07:16 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:00:45 2008''

Revision as of 08:00, 29 July 2008

Image:2ghq.png

Template:STRUCTURE 2ghq

CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II

Template:ABSTRACT PUBMED 17157258

About this Structure

2GHQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1., Zhang Y, Kim Y, Genoud N, Gao J, Kelly JW, Pfaff SL, Gill GN, Dixon JE, Noel JP, Mol Cell. 2006 Dec 8;24(5):759-70. PMID:17157258

Page seeded by OCA on Tue Jul 29 11:00:45 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ghq"
Personal tools