This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2ghw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ghw.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2ghw.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ghw| PDB=2ghw | SCENE= }}
{{STRUCTURE_2ghw| PDB=2ghw | SCENE= }}
-
'''Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R'''
+
===Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R===
-
==Overview==
+
<!--
-
Severe acute respiratory syndrome (SARS) is a newly emerged infectious disease that caused pandemic spread in 2003. The etiological agent of SARS is a novel coronavirus (SARS-CoV). The coronaviral surface spike protein S is a type I transmembrane glycoprotein that mediates initial host binding via the cell surface receptor angiotensin-converting enzyme 2 (ACE2), as well as the subsequent membrane fusion events required for cell entry. Here we report the crystal structure of the S1 receptor binding domain (RBD) in complex with a neutralizing antibody, 80R, at 2.3 A resolution, as well as the structure of the uncomplexed S1 RBD at 2.2 A resolution. We show that the 80R-binding epitope on the S1 RBD overlaps very closely with the ACE2-binding site, providing a rationale for the strong binding and broad neutralizing ability of the antibody. We provide a structural basis for the differential effects of certain mutations in the spike protein on 80R versus ACE2 binding, including escape mutants, which should facilitate the design of immunotherapeutics to treat a future SARS outbreak. We further show that the RBD of S1 forms dimers via an extensive interface that is disrupted in receptor- and antibody-bound crystal structures, and we propose a role for the dimer in virus stability and infectivity.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16954221}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16954221 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16954221}}
==About this Structure==
==About this Structure==
-
2GHW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHW OCA].
+
2GHW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHW OCA].
==Reference==
==Reference==
Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16954221 16954221]
Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16954221 16954221]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Human sars coronavirus]]
+
[[Category: Sars coronavirus]]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Farzan, M.]]
[[Category: Farzan, M.]]
Line 35: Line 39:
[[Category: S protein]]
[[Category: S protein]]
[[Category: Sar]]
[[Category: Sar]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:07:45 2008''
+
[[Category: Virus/viral protein/antibiotic complex]]
 +
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 9 10:04:56 2008''

Revision as of 07:04, 9 July 2008

Image:2ghw.png

Template:STRUCTURE 2ghw

Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R

Template:ABSTRACT PUBMED 16954221

About this Structure

2GHW is a Single protein structure of sequence from Homo sapiens and Sars coronavirus. Full crystallographic information is available from OCA.

Reference

Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:16954221

Page seeded by OCA on Wed Jul 9 10:04:56 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ghw"
Personal tools