2gim

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{{STRUCTURE_2gim| PDB=2gim | SCENE= }}
{{STRUCTURE_2gim| PDB=2gim | SCENE= }}
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'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''
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===1.6 Angstrom structure of plastocyanin from Anabaena variabilis===
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==Overview==
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Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.
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(as it appears on PubMed at http://www.pubmed.gov), where 16929103 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16929103}}
==About this Structure==
==About this Structure==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:25:11 2008''

Revision as of 08:25, 29 July 2008

Image:2gim.png

Template:STRUCTURE 2gim

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

Template:ABSTRACT PUBMED 16929103

About this Structure

2GIM is a Single protein structure of sequence from Anabaena variabilis. Full crystallographic information is available from OCA.

Reference

Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103

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