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| - | [[Image:2gix.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2gix| PDB=2gix | SCENE= }} | | {{STRUCTURE_2gix| PDB=2gix | SCENE= }} |
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| - | '''Cytoplasmic Domain Structure of Kir2.1 containing Andersen's Mutation R218Q and Rescue Mutation T309K'''
| + | ===Cytoplasmic Domain Structure of Kir2.1 containing Andersen's Mutation R218Q and Rescue Mutation T309K=== |
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| - | ==Overview==
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| - | Kir2.1 channels play a key role in maintaining the correct resting potential in eukaryotic cells. Recently, specific amino acid mutations in the Kir2.1 inwardly rectifying potassium channel have been found to cause Andersen's Syndrome in humans. Here, we have characterized individual Andersen's Syndrome mutants R218Q, G300V, E303K, and delta314-315 and have found multiple effects on the ability of the cytoplasmic domains in Kir2.1 channels to form proper tetrameric assemblies. For the R218Q mutation, we identified a second site mutation (T309K) that restored tetrameric assembly but not function. We successfully crystallized and solved the structure (at 2.0 A) of the N- and C-terminal cytoplasmic domains of Kir2.1-R218Q/T309K(S). This new structure revealed multiple conformations of the G-loop and CD loop, providing an explanation for channels that assemble but do not conduct ions. Interestingly, Glu303 forms both intra- and intersubunit salt bridges, depending on the conformation of the G-loop, suggesting that the E303K mutant stabilizes both closed and open G-loop conformations. In the Kir2.1-R218Q/T309K(S) structure, we discovered that the DE loop forms a hydrophobic pocket that binds 2-methyl-2,4-pentanediol, which is located near the putative G(betagamma)-activation site of Kir3 channels. Finally, we observed a potassium ion bound to the cytoplasmic domain for this class of K+ channels.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16834334}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16834334 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16834334}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Andersen's mutation]] | | [[Category: Andersen's mutation]] |
| | [[Category: Cytoplasmic domains of kir2 1]] | | [[Category: Cytoplasmic domains of kir2 1]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:09:30 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:30:53 2008'' |
Revision as of 12:30, 29 July 2008
Template:STRUCTURE 2gix
Cytoplasmic Domain Structure of Kir2.1 containing Andersen's Mutation R218Q and Rescue Mutation T309K
Template:ABSTRACT PUBMED 16834334
About this Structure
2GIX is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Andersen's syndrome mutation effects on the structure and assembly of the cytoplasmic domains of Kir2.1., Pegan S, Arrabit C, Slesinger PA, Choe S, Biochemistry. 2006 Jul 18;45(28):8599-606. PMID:16834334
Page seeded by OCA on Tue Jul 29 15:30:53 2008
