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2gj3

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{{STRUCTURE_2gj3| PDB=2gj3 | SCENE= }}
{{STRUCTURE_2gj3| PDB=2gj3 | SCENE= }}
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'''Crystal structure of the FAD-containing PAS domain of the protein NifL from Azotobacter vinelandii.'''
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===Crystal structure of the FAD-containing PAS domain of the protein NifL from Azotobacter vinelandii.===
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==Overview==
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NifL is a multidomain sensor protein responsible for the transcriptional regulation of genes involved in response to changes in cellular redox state and ADP concentration. Cellular redox is monitored by the N-terminal PAS domain of NifL which contains an FAD cofactor. Flavin-based PAS domains of this type have also been referred to as LOV domains. To explore the mechanism of signal recognition and transduction in NifL, we determined the crystal structure of the FAD-bound PAS domain of NifL from Azotobacter vinelandii to 1.04 A resolution. The structure reveals a novel cavity within the PAS domain which contains two water molecules directly coordinated to the FAD. This cavity is connected to solvent by multiple access channels which may facilitate the oxidation of the FAD by molecular oxygen and the release of hydrogen peroxide. The structure contains a dimer of the NifL PAS domain that is structurally very similar to those described in other crystal structures of PAS domains and identifies a conserved dimerization motif. An N-terminal amphipathic helix constitutes part of the dimerization interface, and similar N-terminal helices are identified in other PAS domain proteins. The structure suggests a model for redox-mediated signaling in which a conformational change is initiated by redox-dependent changes in protonation at the N5 atom of FAD that lead to reorganization of hydrogen bonds within the flavin binding pocket. A structural signal is subsequently transmitted to the beta-sheet interface between the monomers of the PAS domain.
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(as it appears on PubMed at http://www.pubmed.gov), where 17319691 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17319691}}
==About this Structure==
==About this Structure==
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[[Category: Pas domain]]
[[Category: Pas domain]]
[[Category: Redox sensor]]
[[Category: Redox sensor]]
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Revision as of 07:35, 28 July 2008

Image:2gj3.png

Template:STRUCTURE 2gj3

Crystal structure of the FAD-containing PAS domain of the protein NifL from Azotobacter vinelandii.

Template:ABSTRACT PUBMED 17319691

About this Structure

2GJ3 is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.

Reference

Structure of the redox sensor domain of Azotobacter vinelandii NifL at atomic resolution: signaling, dimerization, and mechanism., Key J, Hefti M, Purcell EB, Moffat K, Biochemistry. 2007 Mar 27;46(12):3614-23. Epub 2007 Feb 24. PMID:17319691

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