2gjl

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{{STRUCTURE_2gjl| PDB=2gjl | SCENE= }}
{{STRUCTURE_2gjl| PDB=2gjl | SCENE= }}
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'''Crystal Structure of 2-nitropropane dioxygenase'''
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===Crystal Structure of 2-nitropropane dioxygenase===
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==Overview==
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Nitroalkane compounds are widely used in chemical industry and are also produced by microorganisms and plants. Some nitroalkanes have been demonstrated to be carcinogenic, and enzymatic oxidation of nitroalkanes is of considerable interest. 2-Nitropropane dioxygenases from Neurospora crassa and Williopsis mrakii (Hansenula mrakii), members of one family of the nitroalkane-oxidizing enzymes, contain FMN and FAD, respectively. The enzymatic oxidation of nitroalkanes by 2-nitropropane dioxygenase operates by an oxidase-style catalytic mechanism, which was recently shown to involve the formation of an anionic flavin semiquinone. This represents a unique case in which an anionic flavin semiquinone has been experimentally observed in the catalytic pathway for oxidation catalyzed by a flavin-dependent enzyme. Here we report the first crystal structure of 2-nitropropane dioxygenase from Pseudomonas aeruginosa in two forms: a binary complex with FMN and a ternary complex with both FMN and 2-nitropropane. The structure identifies His(152) as the proposed catalytic base, thus providing a structural framework for a better understanding of the catalytic mechanism.
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{{ABSTRACT_PUBMED_16682407}}
==About this Structure==
==About this Structure==
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[[Category: 2-nitropropane]]
[[Category: 2-nitropropane]]
[[Category: Fmn]]
[[Category: Fmn]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:10:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:35:12 2008''

Revision as of 17:35, 27 July 2008

Image:2gjl.png

Template:STRUCTURE 2gjl

Crystal Structure of 2-nitropropane dioxygenase

Template:ABSTRACT PUBMED 16682407

About this Structure

2GJL is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base., Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW, J Biol Chem. 2006 Jul 7;281(27):18660-7. Epub 2006 May 8. PMID:16682407

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