2gjw

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{{STRUCTURE_2gjw| PDB=2gjw | SCENE= }}
{{STRUCTURE_2gjw| PDB=2gjw | SCENE= }}
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'''RNA Recognition and Cleavage by an Splicing Endonuclease'''
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===RNA Recognition and Cleavage by an Splicing Endonuclease===
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==Overview==
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The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.
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{{ABSTRACT_PUBMED_16690865}}
==About this Structure==
==About this Structure==
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[[Category: Bulge-helix-bulge rna-protein complex]]
[[Category: Bulge-helix-bulge rna-protein complex]]
[[Category: Splicing endonuclease af]]
[[Category: Splicing endonuclease af]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:11:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:45:44 2008''

Revision as of 16:45, 28 July 2008

Image:2gjw.png

Template:STRUCTURE 2gjw

RNA Recognition and Cleavage by an Splicing Endonuclease

Template:ABSTRACT PUBMED 16690865

About this Structure

2GJW is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:16690865

Page seeded by OCA on Mon Jul 28 19:45:44 2008

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