2gn2

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{{STRUCTURE_2gn2| PDB=2gn2 | SCENE= }}
{{STRUCTURE_2gn2| PDB=2gn2 | SCENE= }}
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'''Crystal structure of tetrameric biodegradative threonine deaminase (TdcB) from Salmonella typhimurium in complex with CMP at 2.5A resolution (Hexagonal form)'''
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===Crystal structure of tetrameric biodegradative threonine deaminase (TdcB) from Salmonella typhimurium in complex with CMP at 2.5A resolution (Hexagonal form)===
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==Overview==
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Two different pyridoxal 5'-phosphate-containing l-threonine deaminases (EC 4.3.1.19), biosynthetic and biodegradative, which catalyze the deamination of l-threonine to alpha-ketobutyrate, are present in Escherichia coli and Salmonella typhimurium. Biodegradative threonine deaminase (TdcB) catalyzes the first reaction in the anaerobic breakdown of l-threonine to propionate. TdcB, unlike the biosynthetic threonine deaminase, is insensitive to l-isoleucine and is activated by AMP. In the present study, TdcB from S. typhimurium was cloned and overexpressed in E. coli. In the presence of AMP or CMP, the recombinant enzyme was converted to the tetrameric form accompanied by significant enzyme activation. To provide insights into ligand-mediated oligomerization and enzyme activation, crystal structures of S. typhimurium TdcB and its complex with CMP were determined. In the native structure, TdcB is in a dimeric form, whereas in the TdcB.CMP complex, it exists in a tetrameric form with 222 symmetry and appears as a dimer of dimers. Tetrameric TdcB binds to four molecules of CMP, two at each of the dimer interfaces. Comparison of the dimer structure in the ligand (CMP)-free and -bound forms suggests that the changes induced by ligand binding at the dimer interface are essential for tetramerization. The differences observed in the tertiary and quaternary structures of TdcB in the absence and presence of CMP appear to account for enzyme activation and increased binding affinity for l-threonine. Comparison of TdcB with related pyridoxal 5'-phosphate-dependent enzymes points to structural and mechanistic similarities.
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(as it appears on PubMed at http://www.pubmed.gov), where 17046821 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17046821}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation., Simanshu DK, Savithri HS, Murthy MR, J Biol Chem. 2006 Dec 22;281(51):39630-41. Epub 2006 Oct 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17046821 17046821]
Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation., Simanshu DK, Savithri HS, Murthy MR, J Biol Chem. 2006 Dec 22;281(51):39630-41. Epub 2006 Oct 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17046821 17046821]
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Crystallization and preliminary X-ray crystallographic analysis of biodegradative threonine deaminase (TdcB) from Salmonella typhimurium., Simanshu DK, Chittori S, Savithri HS, Murthy MR, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):275-8. Epub 2006 Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511321 16511321]
[[Category: Salmonella typhimurium]]
[[Category: Salmonella typhimurium]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Tdcb]]
[[Category: Tdcb]]
[[Category: Threonine dehydratase]]
[[Category: Threonine dehydratase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:17:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:20:05 2008''

Revision as of 03:20, 29 July 2008

Image:2gn2.png

Template:STRUCTURE 2gn2

Crystal structure of tetrameric biodegradative threonine deaminase (TdcB) from Salmonella typhimurium in complex with CMP at 2.5A resolution (Hexagonal form)

Template:ABSTRACT PUBMED 17046821

About this Structure

2GN2 is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation., Simanshu DK, Savithri HS, Murthy MR, J Biol Chem. 2006 Dec 22;281(51):39630-41. Epub 2006 Oct 17. PMID:17046821

Crystallization and preliminary X-ray crystallographic analysis of biodegradative threonine deaminase (TdcB) from Salmonella typhimurium., Simanshu DK, Chittori S, Savithri HS, Murthy MR, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):275-8. Epub 2006 Feb 24. PMID:16511321

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