2gub

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{{STRUCTURE_2gub| PDB=2gub | SCENE= }}
{{STRUCTURE_2gub| PDB=2gub | SCENE= }}
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'''Crystal Structure of Metal Free D-Xylose Isomerase.'''
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===Crystal Structure of Metal Free D-Xylose Isomerase.===
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==Overview==
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Time-of-flight neutron diffraction has been used to locate hydrogen atoms that define the ionization states of amino acids in crystals of D-xylose isomerase. This enzyme, from Streptomyces rubiginosus, is one of the largest enzymes studied to date at high resolution (1.8 A) by this method. We have determined the position and orientation of a metal ion-bound water molecule that is located in the active site of the enzyme; this water has been thought to be involved in the isomerization step in which D-xylose is converted to D-xylulose or D-glucose to D-fructose. It is shown to be water (rather than a hydroxyl group) under the conditions of measurement (pH 8.0). Our analyses also reveal that one lysine probably has an -NH(2)-terminal group (rather than NH(3)(+)). The ionization state of each histidine residue also was determined. High-resolution x-ray studies (at 0.94 A) indicate disorder in some side chains when a truncated substrate is bound and suggest how some side chains might move during catalysis. This combination of time-of-flight neutron diffraction and x-ray diffraction can contribute greatly to the elucidation of enzyme mechanisms.
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(as it appears on PubMed at http://www.pubmed.gov), where 16707576 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16707576}}
==About this Structure==
==About this Structure==
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[[Category: Sugar interconversion]]
[[Category: Sugar interconversion]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:32:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:45:50 2008''

Revision as of 04:45, 28 July 2008

Image:2gub.png

Template:STRUCTURE 2gub

Crystal Structure of Metal Free D-Xylose Isomerase.

Template:ABSTRACT PUBMED 16707576

About this Structure

2GUB is a Single protein structure of sequence from Streptomyces rubiginosus. Full crystallographic information is available from OCA.

Reference

Locating active-site hydrogen atoms in D-xylose isomerase: time-of-flight neutron diffraction., Katz AK, Li X, Carrell HL, Hanson BL, Langan P, Coates L, Schoenborn BP, Glusker JP, Bunick GJ, Proc Natl Acad Sci U S A. 2006 May 30;103(22):8342-7. Epub 2006 May 17. PMID:16707576

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