2gw3

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{{STRUCTURE_2gw3| PDB=2gw3 | SCENE= }}
{{STRUCTURE_2gw3| PDB=2gw3 | SCENE= }}
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'''Crystal structure of stony coral fluorescent protein Kaede, green form'''
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===Crystal structure of stony coral fluorescent protein Kaede, green form===
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==Overview==
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A coral fluorescent protein from Trachyphyllia geoffroyi, Kaede, possesses a tripeptide of His62-Tyr63-Gly64, which forms a chromophore with green fluorescence. This chromophore's fluorescence turns red following UV light irradiation. We have previously shown that such photoconversion is achieved by a formal beta-elimination reaction, which results in a cleavage of the peptide bond found between the amide nitrogen and the alpha-carbon at His62. However, the stereochemical arrangement of the chromophore and the precise structural basis for this reaction mechanism previously remained unknown. Here, we report the crystal structures of the green and red form of Kaede at 1.4 A and 1.6 A resolutions, respectively. Our structures depict the cleaved peptide bond in the red form. The chromophore conformations both in the green and red forms are similar, except a well-defined water molecule in the proximity of the His62 imidazole ring in the green form. We propose a molecular mechanism for green-to-red photoconversion, which is assisted by the water molecule.
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(as it appears on PubMed at http://www.pubmed.gov), where 17692334 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17692334}}
==About this Structure==
==About this Structure==
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[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Luminescent protein]]
[[Category: Luminescent protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:36:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:09:54 2008''

Revision as of 00:09, 29 July 2008

Image:2gw3.png

Template:STRUCTURE 2gw3

Crystal structure of stony coral fluorescent protein Kaede, green form

Template:ABSTRACT PUBMED 17692334

About this Structure

2GW3 is a Single protein structure of sequence from Trachyphyllia geoffroyi. Full crystallographic information is available from OCA.

Reference

Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede., Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, Ikura M, J Mol Biol. 2007 Sep 28;372(4):918-26. Epub 2007 Jun 19. PMID:17692334

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