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2gya

From Proteopedia

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[[Image:2gya.gif|left|200px]]
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{{STRUCTURE_2gya| PDB=2gya | SCENE= }}
{{STRUCTURE_2gya| PDB=2gya | SCENE= }}
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'''Structure of the 50S subunit of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056'''
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===Structure of the 50S subunit of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056===
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==Overview==
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In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled E. coli ribosome complex. A cascade of RNA rearrangements propagates from the exit tunnel throughout the large subunit, affecting intersubunit bridges and tRNA positions, which in turn reorient small subunit RNA elements. Elongation arrest could result from the inhibition of mRNA.(tRNAs) translocation, E site tRNA egress, and perhaps translation factor activation at the GTPase-associated center. Our study suggests that the specific secondary and tertiary arrangement of ribosomal RNA provides the basis for internal signal transduction within the ribosome. Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors.
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The line below this paragraph, {{ABSTRACT_PUBMED_16713583}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16713583 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16713583}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements., Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J, Mol Cell. 2006 May 19;22(4):533-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16713583 16713583]
Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements., Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J, Mol Cell. 2006 May 19;22(4):533-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16713583 16713583]
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A model for co-translational translocation: ribosome-regulated nascent polypeptide translocation at the protein-conducting channel., Mitra K, Frank J, FEBS Lett. 2006 Jun 12;580(14):3353-60. Epub 2006 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16714018 16714018]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Signal transduction]]
[[Category: Signal transduction]]
[[Category: Translocation]]
[[Category: Translocation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:39:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:41:06 2008''

Revision as of 20:41, 28 July 2008

Image:2gya.png

Template:STRUCTURE 2gya

Structure of the 50S subunit of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056

Template:ABSTRACT PUBMED 16713583

About this Structure

2GYA is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements., Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J, Mol Cell. 2006 May 19;22(4):533-43. PMID:16713583

A model for co-translational translocation: ribosome-regulated nascent polypeptide translocation at the protein-conducting channel., Mitra K, Frank J, FEBS Lett. 2006 Jun 12;580(14):3353-60. Epub 2006 May 15. PMID:16714018

Page seeded by OCA on Mon Jul 28 23:41:06 2008

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