2h0h
From Proteopedia
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{{STRUCTURE_2h0h| PDB=2h0h | SCENE= }} | {{STRUCTURE_2h0h| PDB=2h0h | SCENE= }} | ||
| - | + | ===Crystal Structure of DsbG K113E mutant=== | |
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| - | ==Overview== | ||
| - | Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7and 2.0-A resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture of both redox forms, suggesting that oxidized DsbG is less stable than the reduced form. This trait would contribute to DsbG isomerase activity, which requires that the active-site Cys residues are kept reduced, regardless of the highly oxidative environment of the periplasm. We propose that a Thr residue that is conserved in the cis-Pro loop of DsbG and DsbC but not found in other Dsb proteins could play a role in this process. Also, the structure of DsbG reveals an unanticipated and surprising feature that may help define its specific role in oxidative protein folding. Thus, the dimensions and surface features of DsbG show a very large and charged binding surface that is consistent with interaction with globular protein substrates having charged surfaces. This finding suggests that, rather than catalyzing disulfide rearrangement in unfolded substrates, DsbG may preferentially act later in the folding process to catalyze disulfide rearrangement in folded or partially folded proteins. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2H0H is a | + | 2H0H is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0H OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:15184683</ref><references group="xtra"/> | |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Bardwell, J C.A.]] | [[Category: Bardwell, J C.A.]] | ||
[[Category: Heras, B.]] | [[Category: Heras, B.]] | ||
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[[Category: Redox-active center]] | [[Category: Redox-active center]] | ||
[[Category: Thioredoxin fold]] | [[Category: Thioredoxin fold]] | ||
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| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:54:15 2009'' | ||
Revision as of 10:54, 16 February 2009
Crystal Structure of DsbG K113E mutant
About this Structure
2H0H is a 2 chains structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
- Heras B, Edeling MA, Schirra HJ, Raina S, Martin JL. Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8876-81. Epub 2004 Jun 7. PMID:15184683 doi:http://dx.doi.org/10.1073/pnas.0402769101
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