2h35

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[[Image:2h35.gif|left|200px]]
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{{STRUCTURE_2h35| PDB=2h35 | SCENE= }}
{{STRUCTURE_2h35| PDB=2h35 | SCENE= }}
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'''Solution structure of Human normal adult hemoglobin'''
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===Solution structure of Human normal adult hemoglobin===
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==Overview==
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So far high-resolution structure determination by nuclear magnetic resonance (NMR) spectroscopy has been limited to proteins &lt;30 kDa, although global fold determination is possible for substantially larger proteins. Here we present a strategy for assigning backbone and side-chain resonances of large proteins without deuteration, with which one can obtain high-resolution structures from (1)H-(1)H distance restraints. The strategy uses information from through-bond correlation experiments to filter intraresidue and sequential correlations from through-space correlation experiments, and then matches the filtered correlations to obtain sequential assignment. We demonstrate this strategy on three proteins ranging from 24 to 65 kDa for resonance assignment and on maltose binding protein (42 kDa) and hemoglobin (65 kDa) for high-resolution structure determination. The strategy extends the size limit for structure determination by NMR spectroscopy to 42 kDa for monomeric proteins and to 65 kDa for differentially labeled multimeric proteins without the need for deuteration or selective labeling.
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(as it appears on PubMed at http://www.pubmed.gov), where 17060917 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17060917}}
==About this Structure==
==About this Structure==
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2H35 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H35 OCA].
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2H35 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H35 OCA].
==Reference==
==Reference==
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[[Category: Hbco some]]
[[Category: Hbco some]]
[[Category: Hemoglobin]]
[[Category: Hemoglobin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:48:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:55:39 2008''

Revision as of 07:55, 28 July 2008

Image:2h35.png

Template:STRUCTURE 2h35

Solution structure of Human normal adult hemoglobin

Template:ABSTRACT PUBMED 17060917

About this Structure

2H35 is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

A new strategy for structure determination of large proteins in solution without deuteration., Xu Y, Zheng Y, Fan JS, Yang D, Nat Methods. 2006 Nov;3(11):931-7. PMID:17060917

Page seeded by OCA on Mon Jul 28 10:55:39 2008

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