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| - | [[Image:2h5f.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2h5f| PDB=2h5f | SCENE= }} | | {{STRUCTURE_2h5f| PDB=2h5f | SCENE= }} |
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| - | '''Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity'''
| + | ===Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity=== |
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| - | ==Overview==
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| - | Boiga dendrophila (mangrove catsnake) is a colubrid snake that lives in Southeast Asian lowland rainforests and mangrove swamps and that preys primarily on birds. We have isolated, purified, and sequenced a novel toxin from its venom, which we named denmotoxin. It is a monomeric polypeptide of 77 amino acid residues with five disulfide bridges. In organ bath experiments, it displayed potent postsynaptic neuromuscular activity and irreversibly inhibited indirectly stimulated twitches in chick biventer cervicis nerve-muscle preparations. In contrast, it induced much smaller and readily reversible inhibition of electrically induced twitches in mouse hemidiaphragm nerve-muscle preparations. More precisely, the chick muscle alpha(1)betagammadelta-nicotinic acetylcholine receptor was 100-fold more susceptible compared with the mouse receptor. These data indicate that denmotoxin has a bird-specific postsynaptic activity. We chemically synthesized denmotoxin, crystallized it, and solved its crystal structure at 1.9 A by the molecular replacement method. The toxin structure adopts a non-conventional three-finger fold with an additional (fifth) disulfide bond in the first loop and seven additional residues at its N terminus, which is blocked by a pyroglutamic acid residue. This is the first crystal structure of a three-finger toxin from colubrid snake venom and the first fully characterized bird-specific toxin. Denmotoxin illustrates the relationship between toxin specificity and the primary prey type that constitutes the snake's diet.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16864572}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16864572 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16864572}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Snake venom]] | | [[Category: Snake venom]] |
| | [[Category: Three-finger toxin]] | | [[Category: Three-finger toxin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:53:18 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:12:39 2008'' |
Revision as of 00:12, 29 July 2008
Template:STRUCTURE 2h5f
Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity
Template:ABSTRACT PUBMED 16864572
About this Structure
2H5F is a Single protein structure of sequence from Boiga dendrophila. Full crystallographic information is available from OCA.
Reference
Denmotoxin, a three-finger toxin from the colubrid snake Boiga dendrophila (Mangrove Catsnake) with bird-specific activity., Pawlak J, Mackessy SP, Fry BG, Bhatia M, Mourier G, Fruchart-Gaillard C, Servent D, Menez R, Stura E, Menez A, Kini RM, J Biol Chem. 2006 Sep 29;281(39):29030-41. Epub 2006 Jul 24. PMID:16864572
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