2h8l

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{{STRUCTURE_2h8l| PDB=2h8l | SCENE= }}
{{STRUCTURE_2h8l| PDB=2h8l | SCENE= }}
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'''Crystal structure of the bb' fragment of ERp57'''
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===Crystal structure of the bb' fragment of ERp57===
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==Overview==
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The synthesis of proteins in the endoplasmic reticulum (ER) is limited by the rate of correct disulfide bond formation. This process is carried out by protein disulfide isomerases, a family of ER proteins which includes general enzymes such as PDI that recognize unfolded proteins and others that are selective for specific proteins or classes. Using small-angle X-ray scattering and X-ray crystallography, we report the structure of a selective isomerase, ERp57, and its interactions with the lectin chaperone calnexin. Using isothermal titration calorimetry and NMR spectroscopy, we show that the b' domain of ERp57 binds calnexin with micromolar affinity through a conserved patch of basic residues. Disruption of this binding site by mutagenesis abrogates folding of RNase B in an in vitro assay. The relative positions of the ERp57 catalytic sites and calnexin binding site suggest that activation by calnexin is due to substrate recruitment rather than a direct stimulation of ERp57 oxidoreductase activity.
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(as it appears on PubMed at http://www.pubmed.gov), where 16905107 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16905107}}
==About this Structure==
==About this Structure==
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[[Category: Schrag, J D.]]
[[Category: Schrag, J D.]]
[[Category: Thioredoxin-like fold]]
[[Category: Thioredoxin-like fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:59:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:45:18 2008''

Revision as of 16:45, 28 July 2008

Image:2h8l.png

Template:STRUCTURE 2h8l

Crystal structure of the bb' fragment of ERp57

Template:ABSTRACT PUBMED 16905107

About this Structure

2H8L is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the bb' domains of the protein disulfide isomerase ERp57., Kozlov G, Maattanen P, Schrag JD, Pollock S, Cygler M, Nagar B, Thomas DY, Gehring K, Structure. 2006 Aug;14(8):1331-9. PMID:16905107

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