2p2r
From Proteopedia
(New page: 200px<br /> <applet load="2p2r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2p2r, resolution 1.60Å" /> '''Crystal structure o...) |
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caption="2p2r, resolution 1.60Å" /> | caption="2p2r, resolution 1.60Å" /> | ||
'''Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA'''<br /> | '''Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2P2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CYT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2P2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CYT:'>CYT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P2R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:55:36 2008'' |
Revision as of 12:55, 23 January 2008
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Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA
Overview
KH (hnRNP K homology) domains, consisting of approximately 70 amino acid, residues, are present in a variety of nucleic-acid-binding proteins. Among, these are poly(C)-binding proteins (PCBPs), which are important regulators, of mRNA stability and posttranscriptional regulation in general. All PCBPs, contain three different KH domains and recognize poly(C)-sequences with, high affinity and specificity. To reveal the molecular basis of, poly(C)-sequence recognition, we have determined the crystal structure, at, 1.6 A resolution, of PCBP2 KH3 domain in complex with a 7-nt DNA sequence, (5'-AACCCTA-3') corresponding to one repeat of the C-rich strand of human, telomeric DNA. The domain assumes a type-I KH fold in a, betaalphaalphabetabetaalpha configuration. The protein-DNA interface could, be studied in unprecedented detail and is made up of a series of direct, and water-mediated hydrogen bonds between the protein and the DNA, revealing an especially dense network involving several structural water, molecules for the last 2 nt in the core recognition sequence. Unlike, published KH domain structures, the protein crystallizes without, protein-protein contacts, yielding new insights into the dimerization, properties of different KH domains. A nucleotide platform, an interesting, feature found in some RNA molecules, was identified, evidently for the, first time in DNA.
About this Structure
2P2R is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution., Fenn S, Du Z, Lee JK, Tjhen R, Stroud RM, James TL, Nucleic Acids Res. 2007;35(8):2651-60. Epub 2007 Apr 10. PMID:17426136
Page seeded by OCA on Wed Jan 23 14:55:36 2008
Categories: Homo sapiens | Single protein | Du, Z. | Fenn, S. | James, T.L. | Lee, J.K. | Stroud, R.M. | Tjhen, R. | CYT | Protein-dna complex
