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| | {{STRUCTURE_2hg0| PDB=2hg0 | SCENE= }} | | {{STRUCTURE_2hg0| PDB=2hg0 | SCENE= }} |
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| - | '''Structure of the West Nile Viruse envelope glycoprotein'''
| + | ===Structure of the West Nile Viruse envelope glycoprotein=== |
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| - | ==Overview==
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| - | The envelope glycoprotein (E) of West Nile virus (WNV) undergoes a conformational rearrangement triggered by low pH that results in a class II fusion event required for viral entry. Herein we present the 3.0-A crystal structure of the ectodomain of WNV E, which reveals insights into the flavivirus life cycle. We found that WNV E adopts a three-domain architecture that is shared by the E proteins from dengue and tick-borne encephalitis viruses and forms a rod-shaped configuration similar to that observed in immature flavivirus particles. Interestingly, the single N-linked glycosylation site on WNV E is displaced by a novel alpha-helix, which could potentially alter lectin-mediated attachment. The localization of histidines within the hinge regions of E implicates these residues in pH-induced conformational transitions. Most strikingly, the WNV E ectodomain crystallized as a monomer, in contrast to other flavivirus E proteins, which have crystallized as antiparallel dimers. WNV E assembles in a crystalline lattice of perpendicular molecules, with the fusion loop of one E protein buried in a hydrophobic pocket at the DI-DIII interface of another. Dimeric E proteins pack their fusion loops into analogous pockets at the dimer interface. We speculate that E proteins could pivot around the fusion loop-pocket junction, allowing virion conformational transitions while minimizing fusion loop exposure. | + | The line below this paragraph, {{ABSTRACT_PUBMED_16987985}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16987985 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16987985}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Nybakken, G E.]] | | [[Category: Nybakken, G E.]] |
| | [[Category: Virus/viral protein]] | | [[Category: Virus/viral protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:14:55 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:58:27 2008'' |
Revision as of 21:58, 27 July 2008
Template:STRUCTURE 2hg0
Structure of the West Nile Viruse envelope glycoprotein
Template:ABSTRACT PUBMED 16987985
About this Structure
2HG0 is a Single protein structure of sequence from West nile virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the West Nile virus envelope glycoprotein., Nybakken GE, Nelson CA, Chen BR, Diamond MS, Fremont DH, J Virol. 2006 Dec;80(23):11467-74. Epub 2006 Sep 20. PMID:16987985
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