2hhh

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{{STRUCTURE_2hhh| PDB=2hhh | SCENE= }}
{{STRUCTURE_2hhh| PDB=2hhh | SCENE= }}
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'''Crystal structure of kasugamycin bound to the 30S ribosomal subunit'''
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===Crystal structure of kasugamycin bound to the 30S ribosomal subunit===
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==Overview==
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Kasugamycin (Ksg) specifically inhibits translation initiation of canonical but not of leaderless messenger RNAs. Ksg inhibition is thought to occur by direct competition with initiator transfer RNA. The 3.35-A structure of Ksg bound to the 30S ribosomal subunit presented here provides a structural description of two Ksg-binding sites as well as a basis for understanding Ksg resistance. Notably, neither binding position overlaps with P-site tRNA; instead, Ksg mimics codon nucleotides at the P and E sites by binding within the path of the mRNA. Coupled with biochemical experiments, our results suggest that Ksg indirectly inhibits P-site tRNA binding through perturbation of the mRNA-tRNA codon-anticodon interaction during 30S canonical initiation. In contrast, for 70S-type initiation on leaderless mRNA, the overlap between mRNA and Ksg is reduced and the binding of tRNA is further stabilized by the presence of the 50S subunit, minimizing Ksg efficacy.
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==About this Structure==
==About this Structure==
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[[Category: Initiation]]
[[Category: Initiation]]
[[Category: Ribosome]]
[[Category: Ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:17:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:44:07 2008''

Revision as of 17:44, 27 July 2008

Image:2hhh.png

Template:STRUCTURE 2hhh

Crystal structure of kasugamycin bound to the 30S ribosomal subunit

Template:ABSTRACT PUBMED 16998488

About this Structure

2HHH is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation., Schluenzen F, Takemoto C, Wilson DN, Kaminishi T, Harms JM, Hanawa-Suetsugu K, Szaflarski W, Kawazoe M, Shirouzu M, Nierhaus KH, Yokoyama S, Fucini P, Nat Struct Mol Biol. 2006 Oct;13(10):871-8. Epub 2006 Sep 24. PMID:16998488

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