This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2hi3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2hi3.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2hi3.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hi3| PDB=2hi3 | SCENE= }}
{{STRUCTURE_2hi3| PDB=2hi3 | SCENE= }}
-
'''Solution structure of the homeodomain-only protein HOP'''
+
===Solution structure of the homeodomain-only protein HOP===
-
==Overview==
+
<!--
-
Homeodomain-only protein (HOP) is an 8-kDa transcriptional corepressor that is essential for the normal development of the mammalian heart. Previous studies have shown that HOP, which consists entirely of a putative homeodomain, acts downstream of Nkx2.5 and associates with the serum response factor (SRF), repressing transcription from SRF-responsive genes. HOP is also able to recruit histone deacetylase (HDAC) activity, consistent with its ability to repress transcription. Unlike other classic homeodomain proteins, HOP does not appear to interact with DNA, although it has been unclear if this is because of an overall divergent structure or because of specific amino acid differences between HOP and other homeodomains. To work toward an understanding of HOP function, we have determined the 3D structure of full-length HOP and used a range of biochemical assays to define the parts of the protein that are functionally important for its repression activity. We show that HOP forms a classical homeodomain fold but that it cannot recognize double stranded DNA, a result that emphasizes the importance of caution in predicting protein function from sequence homology alone. We also demonstrate that two distinct regions on the surface of HOP are required for its ability to repress an SRF-driven reporter gene, and it is likely that these motifs direct interactions between HOP and partner proteins such as SRF- and HDAC-containing complexes. Our results demonstrate that the homeodomain fold has been co-opted during evolution for functions other than sequence-specific DNA binding and suggest that HOP functions as an adaptor protein to mediate transcriptional repression.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16939210}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16939210 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16939210}}
==About this Structure==
==About this Structure==
-
2HI3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HI3 OCA].
+
2HI3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HI3 OCA].
==Reference==
==Reference==
Line 28: Line 32:
[[Category: Yung, W W.]]
[[Category: Yung, W W.]]
[[Category: Homeodomain]]
[[Category: Homeodomain]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:19:18 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:15:53 2008''

Revision as of 12:15, 29 July 2008

Image:2hi3.png

Template:STRUCTURE 2hi3

Solution structure of the homeodomain-only protein HOP

Template:ABSTRACT PUBMED 16939210

About this Structure

2HI3 is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

Analysis of the structure and function of the transcriptional coregulator HOP., Kook H, Yung WW, Simpson RJ, Kee HJ, Shin S, Lowry JA, Loughlin FE, Yin Z, Epstein JA, Mackay JP, Biochemistry. 2006 Sep 5;45(35):10584-90. PMID:16939210

Page seeded by OCA on Tue Jul 29 15:15:53 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hi3"
Personal tools