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2hl7

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{{STRUCTURE_2hl7| PDB=2hl7 | SCENE= }}
{{STRUCTURE_2hl7| PDB=2hl7 | SCENE= }}
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'''Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa'''
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===Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa===
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==Overview==
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CcmH (cytochromes c maturation protein H) is an essential component of the assembly line necessary for the maturation of c-type cytochromes in the periplasm of Gram-negative bacteria. The protein is a membrane-anchored thiol-oxidoreductase that has been hypothesized to be involved in the recognition and reduction of apocytochrome c, a prerequisite for covalent heme attachment. Here, we present the 1.7A crystal structure of the soluble periplasmic domain of CcmH from the opportunistic pathogen Pseudomonas aeruginosa (Pa-CcmH*). The protein contains a three-helix bundle, i.e. a fold that is different from that of all other thiol-oxidoreductases reported so far. The catalytic Cys residues of the conserved LRCXXC motif (Cys(25) and Cys(28)), located in a long loop connecting the first two helices, form a disulfide bond in the oxidized enzyme. We have determined the pK(a) values of these 2 Cys residues of Pa-CcmH* (both &gt;8) and propose a possible mechanistic role for a conserved Ser(36) and a water molecule in the active site. The interaction between Pa-CcmH* and Pa-apocyt c(551) (where cyt c(551) represents cytochrome c(551)) was characterized in vitro following the binding kinetics by stopped-flow using a Trp-containing fluorescent variant of Pa-CcmH* and a dansylated peptide, mimicking the apocytochrome c(551) heme binding motif. The kinetic results show that the protein has a moderate affinity to its apocyt substrate, consistent with the role of Pa-CcmH as an intermediate component of the assembly line for c-type cytochrome biogenesis.
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{{ABSTRACT_PUBMED_17623665}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
A strategic protein in cytochrome c maturation: three-dimensional structure of CcmH and binding to apocytochrome c., Di Matteo A, Gianni S, Schinina ME, Giorgi A, Altieri F, Calosci N, Brunori M, Travaglini-Allocatelli C, J Biol Chem. 2007 Sep 14;282(37):27012-9. Epub 2007 Jul 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17623665 17623665]
A strategic protein in cytochrome c maturation: three-dimensional structure of CcmH and binding to apocytochrome c., Di Matteo A, Gianni S, Schinina ME, Giorgi A, Altieri F, Calosci N, Brunori M, Travaglini-Allocatelli C, J Biol Chem. 2007 Sep 14;282(37):27012-9. Epub 2007 Jul 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17623665 17623665]
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Characterization of the Escherichia coli CcmH protein reveals new insights into the redox pathway required for cytochrome c maturation., Fabianek RA, Hofer T, Thony-Meyer L, Arch Microbiol. 1999 Jan;171(2):92-100. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9914305 9914305]
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AtCCMH, an essential component of the c-type cytochrome maturation pathway in Arabidopsis mitochondria, interacts with apocytochrome c., Meyer EH, Giege P, Gelhaye E, Rayapuram N, Ahuja U, Thony-Meyer L, Grienenberger JM, Bonnard G, Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16113-8. Epub 2005 Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16236729 16236729]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Three-helices bundle]]
[[Category: Three-helices bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:04:53 2008''

Revision as of 09:05, 28 July 2008

Image:2hl7.png

Template:STRUCTURE 2hl7

Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa

Template:ABSTRACT PUBMED 17623665

About this Structure

2HL7 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

A strategic protein in cytochrome c maturation: three-dimensional structure of CcmH and binding to apocytochrome c., Di Matteo A, Gianni S, Schinina ME, Giorgi A, Altieri F, Calosci N, Brunori M, Travaglini-Allocatelli C, J Biol Chem. 2007 Sep 14;282(37):27012-9. Epub 2007 Jul 10. PMID:17623665

Characterization of the Escherichia coli CcmH protein reveals new insights into the redox pathway required for cytochrome c maturation., Fabianek RA, Hofer T, Thony-Meyer L, Arch Microbiol. 1999 Jan;171(2):92-100. PMID:9914305

AtCCMH, an essential component of the c-type cytochrome maturation pathway in Arabidopsis mitochondria, interacts with apocytochrome c., Meyer EH, Giege P, Gelhaye E, Rayapuram N, Ahuja U, Thony-Meyer L, Grienenberger JM, Bonnard G, Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16113-8. Epub 2005 Oct 18. PMID:16236729

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