2hlv

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{{STRUCTURE_2hlv| PDB=2hlv | SCENE= }}
{{STRUCTURE_2hlv| PDB=2hlv | SCENE= }}
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'''Bovine Odorant Binding Protein deswapped triple mutant'''
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===Bovine Odorant Binding Protein deswapped triple mutant===
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==Overview==
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The X-ray structure of bovine Odorant Binding Protein (bOBP) revealed its association as a domain swapped dimer. bOBP, devoid of any cysteines, contrasts with other mammalian OBPs, which are monomeric and possess at least one disulfide bridge. We have produced a mutant of bOBP in which a glycine residue was inserted after position 121. This mutation yielded a monomeric bOBP-121Gly+ in which domain swapping has been reverted. Here, we have subsequently introduced two mutations, Trp64Cys and His155Cys, in view to stabilize the putative monomer with a disulfide bridge. We have determined the crystal structure of this triple mutant at 1.65 A resolution. The mutant protein is monomeric, stabilized by a disulfide bridge between Trp64Cys and His155Cys, with a backbone superimposable to that of native bOBP, with the exception of the hinge and of the 10 residues at the C-terminus. bOBP triple mutant binds 1-amino-anthracene, 1-octen-3-ol (bOBP co-purified ligand) and other ligands with muM Kd values comparable to those of the swapped dimer.
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{{ABSTRACT_PUBMED_18269920}}
==About this Structure==
==About this Structure==
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[[Category: Odorant binding protein]]
[[Category: Odorant binding protein]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:26:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:49:46 2008''

Revision as of 09:49, 28 July 2008

Image:2hlv.png

Template:STRUCTURE 2hlv

Bovine Odorant Binding Protein deswapped triple mutant

Template:ABSTRACT PUBMED 18269920

About this Structure

2HLV is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Deswapping bovine odorant binding protein., Ramoni R, Spinelli S, Grolli S, Conti V, Merli E, Cambillau C, Tegoni M, Biochim Biophys Acta. 2008 Apr;1784(4):651-7. Epub 2008 Jan 29. PMID:18269920

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