This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2hm2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2hm2.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2hm2.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hm2| PDB=2hm2 | SCENE= }}
{{STRUCTURE_2hm2| PDB=2hm2 | SCENE= }}
-
'''Solution structure of ASC2'''
+
===Solution structure of ASC2===
-
==Overview==
+
<!--
-
Pyrin domain (PYD)-containing proteins are key components of pathways that regulate inflammation, apoptosis, and cytokine processing. Their importance is further evidenced by the consequences of mutations in these proteins that give rise to autoimmune and hyperinflammatory syndromes. PYDs, like other members of the death domain (DD) superfamily, are postulated to mediate homotypic interactions that assemble and regulate the activity of signaling complexes. However, PYDs are presently the least well characterized of all four DD subfamilies. Here we report the three-dimensional structure and dynamic properties of ASC2, a PYD-only protein that functions as a modulator of multidomain PYD-containing proteins involved in NF-kappaB and caspase-1 activation. ASC2 adopts a six-helix bundle structure with a prominent loop, comprising 13 amino acid residues, between helices two and three. This loop represents a divergent feature of PYDs from other domains with the DD fold. Detailed analysis of backbone 15N NMR relaxation data using both the Lipari-Szabo model-free and reduced spectral density function formalisms revealed no evidence of contiguous stretches of polypeptide chain with dramatically increased internal motion, except at the extreme N and C termini. Some mobility in the fast, picosecond to nanosecond timescale, was seen in helix 3 and the preceding alpha2-alpha3 loop, in stark contrast to the complete disorder seen in the corresponding region of the NALP1 PYD. Our results suggest that extensive conformational flexibility in helix 3 and the alpha2-alpha3 loop is not a general feature of pyrin domains. Further, a transition from complete disorder to order of the alpha2-alpha3 loop upon binding, as suggested for NALP1, is unlikely to be a common attribute of pyrin domain interactions.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16905547}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16905547 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16905547}}
==About this Structure==
==About this Structure==
-
2HM2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HM2 OCA].
+
2HM2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HM2 OCA].
==Reference==
==Reference==
Line 27: Line 31:
[[Category: Pyrin domain]]
[[Category: Pyrin domain]]
[[Category: Six helix bundle]]
[[Category: Six helix bundle]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:26:50 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:06:51 2008''

Revision as of 17:06, 28 July 2008

Image:2hm2.png

Template:STRUCTURE 2hm2

Solution structure of ASC2

Template:ABSTRACT PUBMED 16905547

About this Structure

2HM2 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure and dynamics of ASC2, a pyrin domain-only protein that regulates inflammatory signaling., Natarajan A, Ghose R, Hill JM, J Biol Chem. 2006 Oct 20;281(42):31863-75. Epub 2006 Aug 10. PMID:16905547

Page seeded by OCA on Mon Jul 28 20:06:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hm2"
Personal tools