2hmy

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{{STRUCTURE_2hmy| PDB=2hmy | SCENE= }}
{{STRUCTURE_2hmy| PDB=2hmy | SCENE= }}
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'''BINARY COMPLEX OF HHAI METHYLTRANSFERASE WITH ADOMET FORMED IN THE PRESENCE OF A SHORT NONPSECIFIC DNA OLIGONUCLEOTIDE'''
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===BINARY COMPLEX OF HHAI METHYLTRANSFERASE WITH ADOMET FORMED IN THE PRESENCE OF A SHORT NONPSECIFIC DNA OLIGONUCLEOTIDE===
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==Overview==
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We have determined a structure for a complex formed between HhaI methyltransferase (M.HhaI) and S-adenosyl-L-methionine (AdoMet) in the presence of a non-specific short oligonucleotide. M.HhaI binds to the non-specific short oligonucleotides in solution. Although no DNA is incorporated in the crystal, AdoMet binds in a primed orientation, identical with that observed in the ternary complex of the enzyme, cognate DNA, and AdoMet or S-adenosyl-L-homocysteine (AdoHcy). This orientation differs from the previously observed unprimed orientation in the M.HhaI-AdoMet binary complex, where the S+-CH3 unit of AdoMet is protected by a favorable cation-pi interaction with Trp41. The structure suggests that the presence of DNA can guide AdoMet into the primed orientation. These results shed new light on the proposed ordered mechanism of binding and explains the stable association between AdoMet and M.HhaI.
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{{ABSTRACT_PUBMED_10080885}}
==About this Structure==
==About this Structure==
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[[Category: Roberts, R J.]]
[[Category: Roberts, R J.]]
[[Category: Zhang, X.]]
[[Category: Zhang, X.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:38:26 2008''

Revision as of 11:38, 29 July 2008

Image:2hmy.png

Template:STRUCTURE 2hmy

BINARY COMPLEX OF HHAI METHYLTRANSFERASE WITH ADOMET FORMED IN THE PRESENCE OF A SHORT NONPSECIFIC DNA OLIGONUCLEOTIDE

Template:ABSTRACT PUBMED 10080885

About this Structure

2HMY is a Single protein structure of sequence from Haemophilus haemolyticus. Full crystallographic information is available from OCA.

Reference

Structure of a binary complex of HhaI methyltransferase with S-adenosyl-L-methionine formed in the presence of a short non-specific DNA oligonucleotide., O'Gara M, Zhang X, Roberts RJ, Cheng X, J Mol Biol. 1999 Mar 26;287(2):201-9. PMID:10080885

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