2pw8
From Proteopedia
(New page: 200px<br /> <applet load="2pw8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pw8, resolution 1.840Å" /> '''Crystal structure ...) |
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| - | [[Image:2pw8. | + | [[Image:2pw8.jpg|left|200px]]<br /><applet load="2pw8" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2pw8, resolution 1.840Å" /> | caption="2pw8, resolution 1.840Å" /> | ||
'''Crystal structure of sulfo-hirudin complexed to thrombin'''<br /> | '''Crystal structure of sulfo-hirudin complexed to thrombin'''<br /> | ||
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==Overview== | ==Overview== | ||
The leech-derived anticoagulant hirudin is post-translationally sulfated, on tyrosine 63, resulting in a >10-fold increase in its affinity for, thrombin. We report the structure of a biosynthetic sulfo-hirudin, complexed to thrombin solved to 1.84 A resolution and show that sulfation, is responsible for a salt bridge and an extended hydrogen-bond network, that taken together account for the increased affinity of sulfo-hirudin, for thrombin. We also identify a divalent cation binding site at the, interface between the two subunits of alpha-thrombin that may modulate the, physiological activity of thrombin. | The leech-derived anticoagulant hirudin is post-translationally sulfated, on tyrosine 63, resulting in a >10-fold increase in its affinity for, thrombin. We report the structure of a biosynthetic sulfo-hirudin, complexed to thrombin solved to 1.84 A resolution and show that sulfation, is responsible for a salt bridge and an extended hydrogen-bond network, that taken together account for the increased affinity of sulfo-hirudin, for thrombin. We also identify a divalent cation binding site at the, interface between the two subunits of alpha-thrombin that may modulate the, physiological activity of thrombin. | ||
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| - | ==Disease== | ||
| - | Known diseases associated with this structure: Dysprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]], Hyperprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]], Hypoprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 2PW8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NI and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2PW8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PW8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: thrombin]] | [[Category: thrombin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:33:46 2008'' |
Revision as of 10:33, 23 January 2008
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Crystal structure of sulfo-hirudin complexed to thrombin
Overview
The leech-derived anticoagulant hirudin is post-translationally sulfated, on tyrosine 63, resulting in a >10-fold increase in its affinity for, thrombin. We report the structure of a biosynthetic sulfo-hirudin, complexed to thrombin solved to 1.84 A resolution and show that sulfation, is responsible for a salt bridge and an extended hydrogen-bond network, that taken together account for the increased affinity of sulfo-hirudin, for thrombin. We also identify a divalent cation binding site at the, interface between the two subunits of alpha-thrombin that may modulate the, physiological activity of thrombin.
About this Structure
2PW8 is a Protein complex structure of sequences from Hirudo medicinalis and Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal Structure of a Biosynthetic Sulfo-hirudin Complexed to Thrombin., Liu CC, Brustad E, Liu W, Schultz PG, J Am Chem Soc. 2007 Aug 9;. PMID:17685615
Page seeded by OCA on Wed Jan 23 12:33:46 2008
Categories: Hirudo medicinalis | Homo sapiens | Protein complex | Brustad, E. | Liu, C.C. | Liu, W. | Schultz, P.G. | NA | NI | Hirudin | Hydrolase | Sulfotyrosine | Thrombin
