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2hwg

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{{STRUCTURE_2hwg| PDB=2hwg | SCENE= }}
{{STRUCTURE_2hwg| PDB=2hwg | SCENE= }}
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'''Structure of phosphorylated Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system'''
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===Structure of phosphorylated Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system===
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==Overview==
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Bacterial transport of many sugars, coupled to their phosphorylation, is carried out by the phosphoenolpyruvate (PEP):sugar phosphotransferase system and involves five phosphoryl group transfer reactions. Sugar translocation initiates with the Mg(2+)-dependent phosphorylation of enzyme I (EI) by PEP. Crystals of Escherichia coli EI were obtained by mixing the protein with Mg(2+) and PEP, followed by oxalate, an EI inhibitor. The crystal structure reveals a dimeric protein where each subunit comprises three domains: a domain that binds the partner PEP:sugar phosphotransferase system protein, HPr; a domain that carries the phosphorylated histidine residue, His-189; and a PEP-binding domain. The PEP-binding site is occupied by Mg(2+) and oxalate, and the phosphorylated His-189 is in-line for phosphotransfer to/from the ligand. Thus, the structure represents an enzyme intermediate just after phosphotransfer from PEP and before a conformational transition that brings His-189 approximately P in proximity to the phosphoryl group acceptor, His-15 of HPr. A model of this conformational transition is proposed whereby swiveling around an alpha-helical linker disengages the His domain from the PEP-binding domain. Assuming that HPr binds to the HPr-binding domain as observed by NMR spectroscopy of an EI fragment, a rotation around two linker segments orients the His domain relative to the HPr-binding domain so that His-189 approximately P and His-15 are appropriately stationed for an in-line phosphotransfer reaction.
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{{ABSTRACT_PUBMED_17053069}}
==About this Structure==
==About this Structure==
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[[Category: Phosphoenolpyruvate:sugar phosphotransferase system]]
[[Category: Phosphoenolpyruvate:sugar phosphotransferase system]]
[[Category: Pt]]
[[Category: Pt]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:25:42 2008''

Revision as of 11:25, 27 July 2008

Image:2hwg.png

Template:STRUCTURE 2hwg

Structure of phosphorylated Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system

Template:ABSTRACT PUBMED 17053069

About this Structure

2HWG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of phosphorylated enzyme I, the phosphoenolpyruvate:sugar phosphotransferase system sugar translocation signal protein., Teplyakov A, Lim K, Zhu PP, Kapadia G, Chen CC, Schwartz J, Howard A, Reddy PT, Peterkofsky A, Herzberg O, Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16218-23. Epub 2006 Oct 19. PMID:17053069

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