2hwx

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[[Image:2hwx.gif|left|200px]]
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{{Seed}}
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[[Image:2hwx.png|left|200px]]
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{{STRUCTURE_2hwx| PDB=2hwx | SCENE= }}
{{STRUCTURE_2hwx| PDB=2hwx | SCENE= }}
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'''Structure of human SMG6 E1282C PIN domain mutant.'''
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===Structure of human SMG6 E1282C PIN domain mutant.===
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==Overview==
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SMG6 and SMG5 are essential factors in nonsense-mediated mRNA decay, a conserved pathway that degrades mRNAs with premature translation termination codons. Both SMG5 and SMG6 have been predicted to contain a C-terminal PIN (PilT N-terminus) domain, present in proteins with ribonuclease activity. We have determined the structures of human SMG5 and SMG6 PIN domains. Although they share a similar overall fold related to ribonucleases of the RNase H family, they have local differences at the putative active site. SMG6 has the canonical triad of acidic residues that are crucial in RNase H for nuclease activity, while SMG5 lacks key catalytic residues. The structural differences are reflected at the functional level. Only the PIN domain of SMG6 has degradation activity on single-stranded RNA in vitro. This difference in catalytic activity is conserved in Drosophila, where an SMG6 with an inactive PIN domain inhibits NMD in a dominant-negative manner. Our findings suggest that the NMD machinery has intrinsic nuclease activity that is likely to contribute to the rapid decay of mRNAs that terminate translation prematurely.
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(as it appears on PubMed at http://www.pubmed.gov), where 17053788 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17053788}}
==About this Structure==
==About this Structure==
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[[Category: P body]]
[[Category: P body]]
[[Category: Rna degradation]]
[[Category: Rna degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:48:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:51:53 2008''

Revision as of 23:52, 27 July 2008

Image:2hwx.png

Template:STRUCTURE 2hwx

Structure of human SMG6 E1282C PIN domain mutant.

Template:ABSTRACT PUBMED 17053788

About this Structure

2HWX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillance complex., Glavan F, Behm-Ansmant I, Izaurralde E, Conti E, EMBO J. 2006 Nov 1;25(21):5117-25. Epub 2006 Oct 19. PMID:17053788

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