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2hye

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{{STRUCTURE_2hye| PDB=2hye | SCENE= }}
{{STRUCTURE_2hye| PDB=2hye | SCENE= }}
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'''Crystal Structure of the DDB1-Cul4A-Rbx1-SV5V Complex'''
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===Crystal Structure of the DDB1-Cul4A-Rbx1-SV5V Complex===
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==Overview==
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Protein ubiquitination is a common form of post-translational modification that regulates a broad spectrum of protein substrates in diverse cellular pathways. Through a three-enzyme (E1-E2-E3) cascade, the attachment of ubiquitin to proteins is catalysed by the E3 ubiquitin ligase, which is best represented by the superfamily of the cullin-RING complexes. Conserved from yeast to human, the DDB1-CUL4-ROC1 complex is a recently identified cullin-RING ubiquitin ligase, which regulates DNA repair, DNA replication and transcription, and can also be subverted by pathogenic viruses to benefit viral infection. Lacking a canonical SKP1-like cullin adaptor and a defined substrate recruitment module, how the DDB1-CUL4-ROC1 E3 apparatus is assembled for ubiquitinating various substrates remains unclear. Here we present crystallographic analyses of the virally hijacked form of the human DDB1-CUL4A-ROC1 machinery, which show that DDB1 uses one beta-propeller domain for cullin scaffold binding and a variably attached separate double-beta-propeller fold for substrate presentation. Through tandem-affinity purification of human DDB1 and CUL4A complexes followed by mass spectrometry analysis, we then identify a novel family of WD40-repeat proteins, which directly bind to the double-propeller fold of DDB1 and serve as the substrate-recruiting module of the E3. Together, our structural and proteomic results reveal the structural mechanisms and molecular logic underlying the assembly and versatility of a new family of cullin-RING E3 complexes.
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(as it appears on PubMed at http://www.pubmed.gov), where 16964240 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16964240}}
==Disease==
==Disease==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:35:58 2008''

Revision as of 17:36, 27 July 2008

Image:2hye.png

Template:STRUCTURE 2hye

Contents

Crystal Structure of the DDB1-Cul4A-Rbx1-SV5V Complex

Template:ABSTRACT PUBMED 16964240

Disease

Known disease associated with this structure: Xeroderma pigmentosum, group E, subtype 2 OMIM:[600045]

About this Structure

2HYE is a Protein complex structure of sequences from Homo sapiens and Simian virus 5. Full crystallographic information is available from OCA.

Reference

Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery., Angers S, Li T, Yi X, MacCoss MJ, Moon RT, Zheng N, Nature. 2006 Oct 5;443(7111):590-3. PMID:16964240

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